| 吴晓翠 樊红琨 乔鹏 潘小丽 王文玉.放疗与吉西他滨治疗对肺癌A549 细胞自噬相关基因Beclin1表达的影响[J].现代生物医学进展英文版,2015,15(34):6642-6644. |
| 放疗与吉西他滨治疗对肺癌A549 细胞自噬相关基因Beclin1表达的影响 |
| The Effect of Radiotherapy and Gemcitabine on Autophgy-related GeneBeclin1 in Lung Cancer A549 |
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| DOI： |
| 中文关键词: 自噬相关基因Beclin1 肺癌A549细胞 放射治疗 吉西他滨 |
| 英文关键词: Autophagy gene Beclin1 Lung cancer A549 cell Radiotherapy Gemcitabine |
| 基金项目:河南省科技创新人才工程资助项目（0623060900） |
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| 中文摘要: |
| 目的：探讨放疗与吉西他滨（Gemcitabine，GEM）治疗对人肺腺癌A549 细胞中自噬相关基因Beclin1 表达的影响。方法：使用
60Co r照射（6Gy）人肺腺癌A549 细胞，细胞继续培养6、12和24h。使用人剂量吉西他滨处理人肺腺癌A549 细胞，细胞继续培养
24 h 后，以未处理的A549 细胞为对照，用RT-PCR和Western blotting法检测A549 细胞中Beclin1 mRNA和蛋白的表达。结果：
经过放射线照射后，三组A549 细胞内Beclin1 mRNA和蛋白的表达量均增加，且在24小时末表达量达到最大。经过吉西他滨处
理后，吉西他滨处理组A549细胞内Beclin1 mRNA 和蛋白的表达量均增加。结论：放射治疗与吉西他滨治疗均可导致A549 细胞
内自噬相关基因Beclin1 表达上调。 |
| 英文摘要: |
| Objective:To investigate the effect of radiotherapy and Gemcitabine (GEM) on the expression of autophagy-related
gene Beclin1 in human lung adenocarcinoma A549 cells.Methods:Using 60Co gamma irradiation (6 Gy), the human lung
adenocarcinoma A549 cells were continued to develop for 6, 12 and 24 h. The human lung adenocarcinoma A549 cells were treated by
the human dose of gemcitabine and cultured for 24 h. The untreated A549 cells were taken as control. RT-PCR and Western blotting
method were applied to detect the mRNA and protein expressions of Beclin1 in A549 cells.Results:After radiation, the mRNA and
protein expressions of Beclin1 in A549 cells of three groups increased, and achieved maximum at the end of 24 hours. After treated with
gemcitabine, the mRNA and protein expression of Beclin1 also increased in A549 cells of gemcitabine-treated group.Conclusion:Radiation treatment and gemcitabine therapy both could result in upregulation of expression of autophagy related gene Beclin1in A549
cells. |
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