| 田孝祥 闫承慧 刘丹 刘艳霞 刘美丽 韩雅玲△.黄芪水提取物减轻高脂饮食引起的小鼠肥胖[J].现代生物医学进展英文版,2015,15(34):6629-6632. |
| 黄芪水提取物减轻高脂饮食引起的小鼠肥胖 |
| Astragalus Radix Extract Alleviates High Fat Diet Induced Obesity in Mice |
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| DOI： |
| 中文关键词: 黄芪水提取物 肥胖 高脂饮食 过氧化物酶体增殖物激活受体r |
| 英文关键词: Astragalus radix extract Obesity High fat diet Peroxisome proliferator activated receptor -r |
| 基金项目:国家自然科学基金面上项目（81370243） |
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| 中文摘要: |
| 目的：研究黄芪水提取物（Astragalus radix extract, ARE）对高脂饮食（High fat diet, HFD）引起的小鼠肥胖的作用及可能机制。
方法：将30 只C57 BL/6 小鼠随机分为正常喂养组（ND 组，n=10）、高脂喂养组（HFD 组，n=10）和高脂喂养+ 黄芪水提取物处理
组（ARE 组，n=10）。记录三组小鼠体重及食物摄入。在喂养16 周时，对小鼠附睾白色脂肪称重，并进行HE 染色观察脂肪细胞大
小；对小鼠肝脏进行进行HE 染色观察肝脏脂肪变性情况。应用ELISA方法检测血清瘦素及脂联素水平。应用Western Blot检测
脂肪组织过氧化物酶体增殖物激活受体酌（Peroxisome proliferator activated receptor-r, PPAR-r）表达。结果：①与ND组相比，HFD
组体重及热量摄入均显著增加，表明肥胖模型建立成功；ARE 处理组的体重较HFD 组显著下降，但其热量摄入与HFD 组相当。
②与ND组相比，HFD 组白色脂肪组织重量增加、脂肪细胞增大、肝细胞出现显著脂肪变性；ARE 处理组上述指标较HFD 组明显
改善。③与ND组相比，HFD 组瘦素水平升高、脂联素水平下降；ARE 处理组与HFD 组相比，瘦素水平降低、脂联素水平升高。④
与ND组相比，HFD 组PPAR-r表达显著增加，而ARE 处理组较HFD 组PPAR-r表达下降。结论：黄芪水提取物可能通过抑制
PPAR酌减轻高质饮食引起的肥胖。 |
| 英文摘要: |
| Objective:To investigate the effects and possible mechanismof astragalus radix extract (ARE) on high fat diet (HFD)
induced obesity in mice.Methods:A total of 30 C57 BL/6 mice were randomly divided into normal diet (ND) group (n=10), HFD group
(n=10) and HFD+ARE group. Body weight and food intake were recorded. After 16 weeks, epididymal white adipose tissue was
weighted and size of adipocytes was observed by H&E staining; fatty degeneration of liver was assessed by H&E staining. Level of leptin
and adiponectin were assessed by ELISA. Expression level of peroxisome proliferator activated receptor r(PPAR-r) was determined by
Western Blot.Results:(1) Compared to ND group, body weight and calorie intake increased in HFD group, which indicates that model of
obesity was successfully established; ARE group had lowered body weight with similar calorie intake in contrast to HFD group. (2)
Compared to ND group, heavier white adipose tissue, increased adipocyte size and significant fatty degeneration were detected in HFD
group; while the above mentioned indexes were greatly improved in ARE group compared to those in HFD group. (3) Compared to ND
group, increased leptin and decreased adiponectin were found in HFD group; whereas lower leptin and higher adiponectin were found in
ARE group compared to HFD group. (4) Compared to ND group, expression level of PPAR-r significantly increased in HFD group, while
ARE treatment reversed this change.Conclusion:ARE alleviates obesity induced by HFD in mice possibly through inhibiting PPAR-r . |
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