| 廖志军 丁罡 袁翠堂 陈思宇△ 胡薇薇 李静.腹水传代Walker256乳腺癌细胞系建立骨癌痛模型的可行性[J].现代生物医学进展英文版,2015,15(34):6624-6628. |
| 腹水传代Walker256乳腺癌细胞系建立骨癌痛模型的可行性 |
| The Feasibility of Ascites Passaged Walker256 Breast Cancer Cell Lines toEstablish the Model of Bone Cancer Pain |
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| DOI： |
| 中文关键词: Walker256 细胞 大鼠骨癌痛模型 腹水传代 |
| 英文关键词: Walker256 cell The rat model of bone cancer pain Ascites cultured |
| 基金项目:国家自然科学基金青年基金项目（81400905）；上海市科委医学引导项目（12411960600）；
崇明县科学技术发展资金项目（CKY2014-11）；崇明县青年科技启明星项目（CQYL2014-03） |
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| 中文摘要: |
| 目的：探讨应用腹水传代培养Walker 256 大鼠乳腺癌细胞系建立wistar 大鼠胫骨癌痛模型的可行性。方法：将体重
180-200g Wistar 大鼠随机分为三组：正常对照组（Control 组）、假手术组（Fake 组）、接种Walker256 乳腺癌细胞组（Model 组）。
Model组为将含1× 108个/mL Walker 256 大鼠乳腺癌细胞悬液20 uL注入Wistar 大鼠胫骨上段骨髓腔制备的骨癌症疼痛模型。
Fake 组经微量进样器注射等量的生理盐水入骨髓腔；Control 组则不进行手术接种，分别于手术后数天（post-cancer cell
implantation day，PID）PID 0 d、7 d、14 d及21 d摄片检查手术侧胫骨，观察大鼠的疼痛行为学变化，PID 0 d、7 d及14 d行胫骨HE
染色。结果：PID 7 d摄片检查提示骨密度不均一，HE 染色见大量肿瘤细胞浸润、骨小梁破坏，PID 14 d Model 组均与Fake 组、
Control组行为学方面有显著的统计学差异（P<0.01）。结论：应用腹水传代培养Walker 256 大鼠乳腺癌细胞系可以建立wistar 大
鼠胫骨癌痛模型。 |
| 英文摘要: |
| Objective:To investigate The possibility of ascites cultured Walker256 rat breast cancer cell line to establish the model
of tibial cancer pain in Wistar rat.Methods:Wistar rats weighing 180-200g were randomly divided into three groups: normal control
group (Control group), sham group (Fake group), cancer cells inoculated group (Model group). In the model group, 20 滋L Walker 256
breast cancer cell suspension with a cell density of 1 × 108/mL was injected into rat tibia bone marrow cavity. Fake animals were
injected with an equal volume of saline; Control group did not undergo any surgical procedure. The X-ray examing, body weighting and
pain behavior testing were carried on post-cancer cell implantation day (PID) 0, 7, 14 and 21, respectively. The tissue used for HE
staining was removed on PID 0, 7 and 14.Results:In PID 7 d examination showed that the bone density was not uniform, and HE
staining showed a large number of tumor cell infiltration and bone trabecular destruction, in PID 14 d Model group, with Fake group and
Control group had significant difference in pain behavior (P<0.01).Conclusion:A wistar rat model of bone cancer pain has been
successfully established by using ascites cultured Walker 256 rat breast cancer cell line. |
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