| 彭延敏 王婷婷 丁文华 李晗 王媺媞 孙秀佳 崔东红.亚慢性地卓西平诱导的精神分裂样小鼠前额叶和海马脑区MIF蛋白表达的变化[J].现代生物医学进展英文版,2015,15(34):6606-6609. |
| 亚慢性地卓西平诱导的精神分裂样小鼠前额叶和海马脑区MIF蛋白表达的变化 |
| Changes of MIF Protein Expressions in the Prefrontal Cortex andHippocampus of Subchronic Injectionof Dizocilpine Induced Schizophrenia Mice |
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| DOI： |
| 中文关键词: 地卓西平 精神分裂症 巨噬细胞迁移抑制因子 |
| 英文关键词: Dizocilpine schizophrenia MIF |
| 基金项目:国家自然科学基金项目（81171266，81271481）；上海市重性精神病重点实验室（13dz2260500） |
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| 中文摘要: |
| 目的：探讨亚慢性地卓西平(MK-801)诱导的精神分裂样小鼠模型中前额叶和海马脑区巨噬细胞迁移抑制因子(Macrophage
migration inhibitory factor，MIF) 蛋白表达的变化。方法：将24 只7 周龄小鼠随机分为对照组、MK-801 组和MK-801+ 奥氮平
(olanzapine，olz)组(n=8)，三组小鼠分别接受0.9%生理盐水、MK-801(0.6 mg/kg)和MK-801(0.6 mg/kg)+奥氮平(2.5 mg/kg)给药，持
续4 周。小鼠行为学通过旷场试验、社交实验进行评价，免疫印迹法检测小鼠前额叶和海马组织中MIF蛋白的表达。结果：
MK-801 处理后，小鼠活动量增加，社交功能受损，且都能被抗精神分裂症药物奥氮平显著改善。MK-801 组小鼠前额叶皮层中
MIF蛋白表达与对照组比较无明显统计学差异(P>0.05)，而海马脑区中MIF 蛋白表达较对照组明显升高(P＜0.05)；MK-801+奥氮
平组小鼠前额叶皮层中MIF 蛋白表达较MK-801 组无显著变化，而海马脑区中MIF蛋白表达较MK-801 组明显降低(P＜0.05)。
结论：亚慢性给予MK-801诱导的精神分裂样小鼠海马脑区中MIF蛋白水平升高，提示MIF蛋白可能参与MK-801 诱导的精神
分裂样行为。 |
| 英文摘要: |
| Objective:To investigate the changes of MIF protein expressions in prefrontal cortex and hippocampus in the
subchronic injection of dizocilpine (MK-801) induced schizophrenia mice.Methods:24 mice were randomly divided into 3 groups:
control group, MK-801group, and MK-801+ olanzapine group (n=8), treated with saline (0.9%), MK-801(0.6 mg/kg), and MK-801 (0.6
mg/kg) + olanzapine (2.5 mg/kg) for 4 weeks, respectively. Open field and social behavior test were conducted to evaluate the
schizophrenia-like behavior phenotype of the mice. The content of MIF protein in prefrontal cortex and hippocampus was determined by
western blot analysis.Results:The locomotor activity of mice increased and the social function was impaired after treated with MK-801,
which could both be reversed by olanzapine. Compared with control group, MIF protein level was elevated in hippocampus after MK-801
treated mice (P<0.05), while had no significant change in PFC (P>0.05). Compared with MK-801 group, MIF protein level was elevated
in hippocampus in MK-801+olanzapine group (P<0.05), which had no significant change in PFC either (P>0.05).Conclusion:MIF
protein level was elevated in hippocampus of MK-801 induced mice model of schizophrenia, which indicated that MIF may be involved
in MK-801 induced schizophrenia-like behavior in C57BL/6 mice. |
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