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目的：探讨HIF-1琢和COX-2 在溃疡性结肠炎（UC）患者外周血及黏膜组织中表达及意义。方法：87 例UC患者根据临床病情分为活动期（n=63）和缓解期（n=24），同期选取行结肠镜检查无异常健康者40 名作为对照组，利用qRT-PCR 对外周血及黏膜组织标本中HIF-1alpha和COX-2 表达进行检测。结果：活动期UC 患者外周血和黏膜组织中HIF-1alpha mRNA和COX-2 mRNA相对表达量均高于缓解期患者，且均高于对照组，差异均有统计学意义（P<0.05）；活动期UC 患者中，临床病情重型患者外周血及黏膜组织中HIF-1-alpha mRNA 和COX-2 mRNA 相对表达量高于中型及轻型患者，差异均有统计学意义（P<0.05），内镜表现分级Ⅲ 级患者外周血及黏膜组织中HIF-1-alpha mRNA和COX-2 mRNA相对表达量高于Ⅱ级及Ⅰ级患者，差异均有统计学意义（P<0.05）；Pearson 相关分析显示，活动期UC患者外周血及黏膜组织中HIF-1alphamRNA和COX-2 mRNA相对表达量均与Mayo 评分呈正相关（r=0. 592、0.722 和0.694、0.456，P<0.05）；Spearman 相关分析显示，活动期UC 患者外周血及黏膜组织中HIF-1-mRNA 和COX-2 mRNA 相对表达量均与临床病情呈正相关（r=0.804、0.826 和0.752、0.763，P<0.05），且与内镜表现分级呈正相关（r=0.803、0.749和 0.858、0.793，P<0.05）。结论：HIF-1-alpha 和COX-2 在UC患者外周血及黏膜组织中呈高表达，且与患者病情严重程度有关。

英文摘要:

Objective:To investigate the expression and significance of HIF-1alpha and COX-2 in peripheral blood and mucosal tissues of patients with ulcerative colitis (UC).Methods:87 cases of UC patients were divided into active stage (n=63) and remission stage (n=24) according to the clinical condition. In the same time, 40 healthy cases diagnosed normal by colonoscopy were selected as the control group. The expressions of HIF-1-alpha and COX-2 in peripheral blood and mucosal tissue samples were detected by using qRT-PCR.Results:The relative expression levels of HIF-1琢mRNA and COX-2 mRNA in peripheral blood and mucosal tissues of active UC patients were higher than those of UC patients in remission stage, and which were all higher than those of the control group, the differences were statistically significant (P<0.05). Among active UC patients, the relative expression levels of HIF-1琢mRNA and COX-2 mRNA in peripheral blood and mucosal tissues in the patients with severe clinical condition were higher than those of the patients with mediumand mild degree, and the differences were statistically significant (P<0.05), and the relative expression levels of HIF-1琢mRNA and COX-2 mRNA in peripheral blood and mucosal tissues of endoscopic grade Ⅲ patients were higher than those of grade Ⅱ and Ⅰ patients, the differences were statistically significant (P<0.05). Pearson correlation analysis showed that the relative expression levels of HIF-1-alpha mRNA and COX-2 mRNA in peripheral blood and mucosal tissues of active UC patients were positively correlated with the Mayo score (r=0.592, 0.722and 0.694, 0.456, P<0.05). Spearman correlation analysis showed that, the relative expression levels of HIF-1-alpha mRNA and COX-2 mRNA in peripheral blood and mucosal tissues of active UC patients were positively correlated clinical condition (r=0.804, 0.826and 0.752, 0.763, P<0.05), and were positively correlated with the endoscopic findings grade (r=0.803, 0.749 and 0.858, 0.793, P<0.05).Conclusion:The expressions of HIF-1-alpha and COX-2 in peripheral blood and mucosal tissues of UC patients were high, and were related with the severity of the patient's condition.

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