| 胡小平 王志维 邓宏平 夏军 任宗力 胡锐 江万里.Fibulin-5 在主动脉夹层血管壁中表达异常[J].现代生物医学进展英文版,2015,15(32):6248-6250. |
| Fibulin-5 在主动脉夹层血管壁中表达异常 |
| Expression of Fibulin-5 in the Vessel Wall of Aortic Dissection |
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| DOI： |
| 中文关键词: 主动脉夹层 Fibulin-5 蛋白 弹性纤维 病因 |
| 英文关键词: Aortic dissection Fibulin-5 Elastic fibers Etiology |
| 基金项目:中央高校基本科研业务费专项资金项目（2042014kf0117）；湖北省自然科学基金面上项目（2015CFB169，2013CKB031） |
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| 中文摘要: |
| 目的：研究Stanford A型主动脉夹层（aortic dissection，AD)升主动脉和正常升主动脉血管组织中Fibulin-5 表达的差异。方
法：收集Stanford A型AD患者手术中切除的升主动脉血管组织标本12 例（AD 组），多器官捐献患者升主动脉血管12 例（对照
组）。采用EVG 染色观察主动脉中膜弹性纤维形态结构；应用SP免疫组织化学法及Western blot 法对标本组织中的Fibulin-5 进
行检测分析。结果：AD组主动脉中膜弹性纤维形态和排列不规则、破碎、丢失，结构紊乱。免疫组织化学显示Fibulin-5 阳性表达见
于主动脉壁平滑肌细胞胞质中，AD组与对照组比较，Fibulin-5 表达明显较少。Western blot蛋白印迹示AD 组Fibulin-5 表达明显
减少，差异有统计学意义(P＜0.05)。结论：Fibulin-5 在AD 主动脉中膜中表达下调，可能在AD的发生中发挥作用。 |
| 英文摘要: |
| Objective:To study the differential expression of Fibulin-5 between the aorta specimens from the aortic dissection
(AD) and normal controls.Methods:12 ascending dissection aorta specimens (AD group, n=12) from patients of acute Stanford A
dissection were taken during surgery; 12 normal ascending aorta specimens (control group, n=12) were taken from multi-organ donors.
EVG staining was used for observing the pathological changes of elastic fibers in the aortic media. SP immunohistochemistry and
Western blot were used to identify the differential expression of Fibulin-5 between the AD group and the control group.Results:The
morphology and arrangement of elastic fibers in the aortic media were anomalous, and the disappearance, loss and chaos of elastic fibers
were also found in AD group. Immunohistochemistry showed Fibulin-5 positively expressed in the cytoplasmof smooth muscle cells, and
the expression of Fibulin-5 was lower in the AD group than control group. Western blot also showed the expression of Fibulin-5 was
lower in the AD group with statistical significance (P＜0.05).Conclusion:Down-regulation of Fibulin-5 the aortic media of AD indicates
that Fibulin-5 may play a role in the formation of AD. |
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