| 王策 陈忠祥 严心涛 马玉婷 武晓东△.声光可调谐滤波器的细胞生物学研究应用[J].现代生物医学进展英文版,2015,15(30):5965-5969. |
| 声光可调谐滤波器的细胞生物学研究应用 |
| Application of Acousto-optic Tunable Filter in Cell Biology |
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| DOI： |
| 中文关键词: 声光可调谐滤波器 可调谐光源 高光谱成像 细胞分析 |
| 英文关键词: Acousto-optic tunable filters Tunable light source Hyperspectral imaging Cell analysis |
| 基金项目:科技部863项目(2011AA02A106)；苏州市应用基础研究项目（SYG201128，SYG201207） |
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| 中文摘要: |
| 波长选择在荧光光谱仪和显微镜等光学应用中发挥了至关重要的作用。声光可调谐滤波器(AOTF)作为一种电光器件可实
现多光源入射波长、功率的同时调制。在声光可调谐滤波器中，压电换能器结合于二氧化碲或石英晶体产生高频声波，改变晶体
折射率形成周期性分布。该现象在晶体中生成衍射光栅，使以布拉格角正交入射的光束被高效衍射至一阶光束。当改变施加到晶
体的信号频率时将改变折射率变化周期，因此，衍射光的波长随之改变。同时，衍射光强度由施加到晶体的信号振幅决定。本文从
声光可调谐滤波器原理和特点出发，总结了声光可调谐滤波器在细胞生物学研究系统中的应用模型。得益于作用时间短、波长分
辨率高、无振动部件等特性，声光可调谐滤波器提升了多波长光源功率调制能力，使细胞计数系统具备了细胞高光谱成像能力。
所以不仅限于传统细胞生物学研究，包含声光可调谐滤波器件的系统还将在多参数高内涵成像分析、扫描荧光显微术、药物毒理
研究等领域成为有力的研究工具。 |
| 英文摘要: |
| Wavelength selection is of great importance in many areas of optical science, including fluorescence spectroscopy and
microscopy. Acousto-optic tunable filter (AOTF) is an electro-optical device which can modulate intensity and wavelength of multiple
optical sources simultaneously. In an acousto-optic tunable filter, a piezoelectric transducer bonded to a crystal of tellurium dioxide or
quartz generates high-frequency acoustic waves to alter the refraction of crystal in a periodic pattern. This phenomenon leads to the
generation of a diffracted grating so that an orthogonal beam incident at the Bragg angle is diffracted with high efficiency into the first
order beam. With change of signal frequency applied to the crystal, the period of the refraction variation alters, and therefore, the
wavelength of diffracted beamchanged. The intensity of the diffracted beamis determined by the amplitude (power) of the signal applied
to the crystal. In this review, the theory and the characteristics of AOTF are presented. The application models in cell biology research
systems are summarized. Benefiting from many advantages such as short interaction time, high spectral resolution and free of vibrational
components, the acousto-optic tunable filters were used to enhance the modulation capabilities in multi-wavelength light sources and get
hyperspectral images in cell count systems. In the future, the systems which include AOTFs will become invaluable tools not only for
classical cell biological research but also for emerging technologies like multiparameter high-content image analysis, scanning
fluorescence microscopy and drug toxicology and others. |
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