| 张铭 纪玉强 谢梅 王楠 赵朝.长链非编码RNA（lncRNA）在氧化型低密度脂蛋白诱导血管内皮细胞损
伤中表达谱的变化[J].现代生物医学进展英文版,2015,15(30):5816-5820. |
| 长链非编码RNA（lncRNA）在氧化型低密度脂蛋白诱导血管内皮细胞损
伤中表达谱的变化 |
| Expression Profiles of Long Noncoding RNA in Oxidized Low-densityLipoprotein Treated Vascular Endothelial Cell |
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| DOI： |
| 中文关键词: 长链非编码RNA 人脐静脉内皮细胞 氧化型低密度脂蛋白 芯片 |
| 英文关键词: Long noncoding RNA Human umbilical vein endothelial cell Oxidized low-density lipoprotein Microarray |
| 基金项目:国家自然科学基金项目（81470550）；教育部科学技术研究重点项目（212170） |
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| 中文摘要: |
| 目的：探讨长链非编码RNA（long noncoding RNA, lncRNA）在氧化型低密度脂蛋白（oxidized low-density lipoprotein,
ox-LDL）诱导的损伤人脐静脉内皮细胞（human umbilical vein endothelial cells, HUVEC）与正常HUVEC 中表达谱的差异。方法：
采用lncRNA 芯片检测ox-LDL诱导的损伤HUVEC与正常HUVEC 中lncRNA 及mRNA的表达差异，筛选出HUVEC 损伤相
关的lncRNA。结果：相对于正常HUVEC，在ox-LDL诱导的损伤HUVEC中表达上调和下调超过2 倍的lncRNAs 和mRNAs 分
别有139 种和113 种，上调和下调超过4 倍的lncRNAs 和mRNAs 分别有35 种和28 种。结论：与正常HUVEC 比较，ox-LDL诱
导的损伤HUVEC 中lncRNA 的表达谱显著变化，lncRNA 可能在血管内皮细胞损伤过程中发挥一定作用。 |
| 英文摘要: |
| Objective:To explore the expression profile long noncoding RNA (lncRNA) in oxidized low-density lipoprotein
(ox-LDL) treated human umbilical vein endothelial cell (HUVEC) compared with normal HUVEC.Methods:The expression profiles of
lncRNA and mRNA in ox-LDL treated HUVEC compared with normal HUVEC were analyzed through the human lncRNA microarray.Results:141 lncRNAs and 113 mRNAs were identified to be different expression (fold change ≥ 2.0) in ox-LDL treated HUVEC
compared with normal HUVEC. 35 lncRNAs and 28 mRNAs were identified to be different expression (fold change≥ 4.0).Conclusion:Compared with normal HUVEC, there are evidently differentially expressed profiles of lncRNA in ox-LDL treated HUVEC. LncRNA
may play a partial role in endothelial dysfunction and damage. |
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