| 陈冠洁 郭盖 卜淑敏△ 程洋子 杨涵.模拟室内高浓度PM2.5 对大鼠血清和组织VEGF蛋白表达的影响[J].现代生物医学进展英文版,2015,15(29):5617-5620. |
| 模拟室内高浓度PM2.5 对大鼠血清和组织VEGF蛋白表达的影响 |
| Effects of High Concentrations of PM2.5 in Simulated Indoor Public Placeson the Serum Level of VEGF and the Protein Expression of VEGF in Heart,Spleen, and Lung Tissues of Rats |
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| DOI： |
| 中文关键词: 大鼠 模拟公共场所室内高浓度PM2.5 VEGF |
| 英文关键词: Rats PM2.5 VEGF |
| 基金项目:北京市教委长城学者项目（CIT&TCD20130337）；科技创新平台项目（PXM2014-014206-000006）；
北京市大学生本科立项(14414015) |
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| 中文摘要: |
| 目的：采用" 密闭环境熏烟法" 模拟PM2.5 高浓度环境，建立大鼠被动吸烟模型，观察模拟公共场所室内高浓度PM2.5 对
正常大鼠血清血管内皮生长因子（VEGF）以及心、脾、肺三种组织VEGF 蛋白表达的影响。方法：将20 只6 月龄雄性健康Wistar
大鼠按体重分层随机分为实验组和对照组。并且两组大鼠均于相同的环境下（温度，湿度，光照）进行饲养。对照组不做任何处理，
实验组进行为期6 周的密闭环境烟熏。末次烟熏结束24-36 小时内，用ELISA检测两组大鼠血清VEGF 水平的变化，用Western
blot检测心、脾、肺三种组织VEGF蛋白表达的变化。结果：与对照组比较，实验组心、脾VEGF1、2 以及肺VEGF1 蛋白表达水平
均显著下降，而肺VEGF2 和血清VEGF 水平无显著变化。结论：6 周模拟公共场所室内高浓度PM2.5 能显著降低大鼠心、脾、肺
VEGF蛋白表达水平。 |
| 英文摘要: |
| Objective:To establish passive smoking rat model by simulating the high concentration of PM2.5 in indoor public
places, the changes of serum level of VEGF and the protein expression of VEGF in heart, spleen, lung tissues of rats were observed in
this paper.Methods:20 healthy male Wistar 6-month-old rats were randomly divided into two groups, experimental group and control
group. And two groups of rats were feed in the same environment (temperature, humidity, illumination). The experimental group rats
were exposed to passive cigarette smoking 2 hours every day in closed environment for 6 weeks. After 24-36 h of the final treatment, the
protein expression of VEGF in heart, spleen, lung tissues were detected by Western blot and the serum level of VEGF was detected by
ELISA.Results:The protein levels of VEGF1, 2 in heart and spleen and VEGF1 in lung of experimental group were significantly lower
than that in control group. However, the VEGF2 protein expression in lung and the serum level of VEGF had no significant differences.Conclusion:The high concentrations of PM2.5 in simulated indoor public places can reduce the protein expression of VEGF in heart,
spleen and lung of rats. |
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