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目的：研究担载碱性成纤维细胞生长因子（b-FGF）微球复合明胶支架的外形特征、孔径、孔隙率及体外释放动力学，以期构建具有缓释功能、高孔隙率的担载细胞因子的新型复合明胶支架。方法：本文利用冷冻相分离法和S/O/W法先将b-FGF水溶液包裹于PLGA 微球中，然后埋置于明胶溶液中制备为多孔复合明胶支架。分别对微球的形态和复合明胶支架的基本形态、孔径、孔隙率进行表征，通过Elisa 法测定b-FGF在复合明胶支架中的体外释放行为。结果：制备成形态良好的三维复合明胶支架，其孔隙率为82.90%± 1.45%，孔径范围为150~300 um，复合明胶支架中b-FGF 在体外缓慢释放20余天。结论：担载蛋白微球复合明胶支架不仅满足组织工程支架的要求，还能有效缓释细胞因子，为细胞和组织生长提供良好的微环境，为进一步应用于组织工程领域提供了可能。

英文摘要:

Objective:To investigate the characterization, the pore size and the porosity ratio of gelatin scaffold with PLGA microspheres containing basic fibroblast growth factor and its release behavior in vitro of b-FGF, for constructing a new composite gelatin scaffold loaded growth factor with sustain-released and high porosity ratio.Methods:By the frozen phase separation method and S/O/W method b-FGF aqueous solution prepared into PLGA microspheres, then embedded into gelatin-scaffold to formthe final composite scaffold. The morphology of microspheres and gelatin scaffold, the pore size and the porosity ratio of gelatin scaffold were characterized and release kinetic of b-FGF in composite gelatin scaffold was detected by Elisa method in vitro.Results:The composite three-dimensional (3D) scaffold had good morphology with the porosity ratio of 82.90%± 1.45%and the pore sizes ranging from 150 to 300 um. In vitro, b-FGF in composite gelatin scaffold had sustained release over 20 days.Conclusion:The gelatin scaffold with PLGA microspheres containing growth factor is not only satisfied the requirements of tissue engineering scaffolds, but also sustained release growth factor for providing a microenvironment to cells and tissue growth and further application to tissue engineering in future.

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