| 魏红 朱龙 贺梦影 许航 武杰 段小波 谭文.高胆固醇饮食的兔丁酰胆碱酯酶活性与血脂的相关性分析[J].现代生物医学进展英文版,2015,15(29):5601-5605. |
| 高胆固醇饮食的兔丁酰胆碱酯酶活性与血脂的相关性分析 |
| Correlative Analyses between Butyrylcholinesterase Activity and Blood Lipidin High Cholesterol Diet Rabbit |
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| DOI： |
| 中文关键词: 胆固醇 兔 丁酰胆碱酯酶 血脂 |
| 英文关键词: Cholesterol Rabbit Butyrylcholinesterase Blood lipid |
| 基金项目:国家自然科学基金项目（31300940）；中央高校基本科研业务费专项基金项目（2014ZM0053）；
深圳华大基因研究院与华南理工大学" 创新基金项目"（SW20130804） |
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| 中文摘要: |
| 目的：通过建立高血脂动物模型，探索高血脂动物血浆丁酰胆碱酯酶（BuChE）的活性与血脂指标的相关性，为BuChE 活性
作为高血脂疾病标志物的可能性提供参考。方法：雄性新西兰兔14 只，随机分为两组（每组各7 只），即普通饲料组（C 组）和高胆
固醇饲料组（H 组）。连续喂养12 周，每二至三周称重采血，采用Ellman 法检测BuChE活性，按照试剂盒使用说明测定血浆总胆
固醇（TC）、甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）；12 周后，麻醉兔子开胸采集胸主动脉，
经10 %的中性福尔马林固定后进行苏丹Ⅳ脂肪染色。结果：兔子喂食高胆固醇饲料12 周后，H组动脉出现斑块，C 组无斑块形
成；兔子在食用高胆固醇饲料的过程中，血浆TC、LDL-C、HDL-C显著升高(P<0.01)，而BuChE 活性无显著变化（P>0.05）；分析正
常兔子和高血脂兔子血浆BuChE活性与TC、TG、HDL-C、LDL-C 的皮尔森相关系数，发现均无相关性（r<<1，P>0.05）。结论：本项
研究未在正常兔子和高血脂的兔子中发现BuChE 与TC、TG、HDL-C、LDL-C 存在相关性，说明通过喂食兔子高胆固醇饲料建立
的高血脂模型不适合探究BuChE 与TC、TG、HDL-C、LDL-C 的相关性。 |
| 英文摘要: |
| Objective:To compare the correlation between butyrylcholinesterase (BuChE) activity and blood lipid in normal and
hyperlipidemia rabbit and to explore the potential of using BuChE as a biomarker for hyperlipidemia.Methods:14 male New Zealand
rabbits were randomly divided into two groups (7 rabbits each), normal diet group (group C) and high-cholesterol diet group (group H).
All rabbits were fed with either normal diet or high-cholesterol diet for 12 weeks. The body weight of each rabbit was recorded and the
peripheral blood was collected every 2 to 3 weeks. The activity of BuChE was detected with Ellman's assay and the concentration of plasma
total cholesterol (TC), triglyceride (TG), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol
(LDL-C) was analyzed with commercial kits. After 12 weeks, rabbits were anaesthetized with urethane, the aorta thoracicas were taken
out and fixed in 10 % neutral formaldehyde for 24 hours, then stained with SudanⅣ .Results:After 12 weeks, clear atherosclerotic
plaques were found in the aorta thoracicas of high-cholesterol diet group (Group H) but not in that of normal diet group (Group C). With
the progress of high cholesterol diet, the TC, LDL-C and HDL-C level in the plasma of group H was much higher compare to that in
groupC (P<0.01), while BuChE activity remained the same compare to the 0 week. The results of the Pearson correlation indicated that
there was no significant correlation between BuChE activity and TC, TG, HDL-C, LDL-C level (r<<1, P>0.05).Conclusion:The result
of this research showed that there was no significant relationship between BuChE activity and TC, TG, HDL-C, LDL-C level, which suggests
that the cholesterol-fed hyperlipidemia rabbit model is not suitable for the correlation analyses between BuChE activity and TC, TG,
HDL-C and LDL-C. |
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