王龙 周钰泽 王凯 张千峰 窦科峰.根皮素对HepG2肝癌细胞增殖和凋亡影响的实验研究[J].现代生物医学进展英文版,2015,15(28):5447-5450. |
根皮素对HepG2肝癌细胞增殖和凋亡影响的实验研究 |
Effect of Phloretinon Proliferation and Apoptosis of HepG2 Cells |
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DOI: |
中文关键词: 根皮素 肝癌细胞 细胞增殖 细胞凋亡 |
英文关键词: Phloretin Liver cancer cells Cell proliferation Cell apoptosis |
基金项目:国家自然科学基金项目(81101619) |
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中文摘要: |
目的:探讨根皮素对人肝癌HepG2细胞增殖和凋亡的影响。方法:MTT法检测不同浓度(30、40、50 ug/mL)根皮素对肝癌
HepG2 细胞增殖的抑制作用,流式细胞技术检测根皮素对HepG2细胞凋亡的影响,ELISA 法检测不同浓度根皮素干预后细胞中
Bcl-2 和Bax表达的变化,Western blot法检测30 ug/mL根皮素在8,16,24 小时后检测p-AKT 蛋白表达情况。结果:30、40 和50
ug/mL 的根皮素对肝癌HepG2 细胞的增殖均有抑制作用(P<0.01),同时,30、40 和50 ug/mL 的根皮素在24 小时后可诱导
HepG2 细胞发生早期凋亡,凋亡率分别为0.1321± 0.0224, 0.2607± 0.0457, 0.3712± 0.0884(P<0.01);另外,30、40 和50 ug/mL的
根皮素作用24 小时后细胞内Bcl-2 表达降低,Bax 表达增高(P<0.01);最后,30 ug/mL根皮素可以明显减少p-AKT 表达量,这
种作用呈现时间依赖性。结论:根皮素能够抑制肝癌HepG2 细胞的增殖和促进细胞凋亡。 |
英文摘要: |
Objective:To evaluate the effects of phloretin on proliferation and apoptosis of HepG2 cells.Methods:Effects of
phloretin on HepG2 cell proliferation and apoptosis were evaluated by MTT method and flow cytometry instrument, respectively.
Expression of Bcl-2 and Bax protein in HepG2 cell with or without phloretin pretreatment was detected by ELISA method. Expression of
the p-AKT protein in HepG2 cell which were treated with phloretin (30 ug/mL) in 8, 16, 24 hours can be measured by Western Blot.Results:Different doses of phloretin (30, 40 and 50 ug/mL) depressed the proliferation of HepG2 cells (P<0.01). Besides, all dosages of
phloretin (30, 40 and 50 ug/mL) induced cell apoptosis in HepG2 significantly compared to that of control,the relative rate of apoptosis:
0.1321± 0.0224, 0.2607± 0.0457, 0.3712± 0.0884(P<0.01). What was more, 30, 40 and 50 ug/mL phloretin decrease the expression
of Bcl-2 and increased Bax expression, respectively; and phloretin can decrease the expression of the p-AKT.Conclusion:Phloretin
could inhibit proliferation and induced apoptosis on HepG2 cells. |
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