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目的：研究14-3-3theta蛋白在人骨肉瘤中的表达和临床意义。方法：在62 例人骨肉瘤和正常骨组织样本中，通过免疫组化的方法检测14-3-3theta 蛋白的表达情况，并分析其表达与骨肉瘤临床病理学特征间的关系。结果：在62 例骨肉瘤和正常骨组织中，14-3-3theta 蛋白表达的阳性率分别为80.6%和17.7%。统计学分析表明，14-3-3theta 蛋白的表达与骨肉瘤肿瘤大小（P=0.016）、高组织学类型（P=0.001）、高Enneking分级（P=0.047）具有显著相关性，而与骨肉瘤患者的年龄（P=0.901）、性别（P=0.691）、肿瘤部位（P=0.802）、碱性磷酸酶（P=0.884）、血清白蛋白（P=0.822）、血沉（P=0.836）等均无关。Spearman 相关性分析检测发现，14-3-3theta 蛋白高表达与肿瘤大小（r = -0.34, P = 0.034）、高组织学分级（r = -0.27, P = 0.031）、高Enneking 分级（r = -0.05, P = 0.025）呈正相关，而与骨肉瘤患者的年龄、性别、肿瘤部位、碱性磷酸酶和血沉等均无相关性（P > 0.05）。结论：在骨肉瘤组织中，14-3-3theta 作为癌基因表达增加，与骨肉瘤的疾病进展密切相关，14-3-3theta 可以作为骨肉瘤疾病监测的一种分子指标。

英文摘要:

Objective:To investigate the expression and clinical significance of 14-3-3theta protein in human osteosarcoma.Methods:Expression status of 14-3-3theta was detected by immunohistochemistry in 62 cases of human osteosarcoma and normal bone tissues. The correlation between the expression of 14-3-3theta and clinicopathological characteristics of osteosarcoma were analyzed.Results:In 62 cases of osteosarcoma and normal bone tissues, the positive expressions of 14-3-3theta were 80.6% and 17.7%. The chi square test suggested that 14-3-3theta expression was correlated with tumor size（P=0.016）, histologic subtype (P=0.001), Enneking stage (P=0.047)，while it was not correlated with age (P=0.901), sex (P=0.691), site of primary disease (P=0.802), alkaline phosphatase (P=0.884), serum albumin (P=0.822), ESR (P=0.836). Correlation analysis suggested that 14-3-3theta expressions were positively correlated with tumor size (r=-0.34, P=0.034), histologic subtype (r=-0.27, P=0.031), Enneking stage (r=-0.05, P=0.025), while it was negatively correlated with age, sex, site, alkaline phosphatase, serum albumin and ESR (P>0.05).Conclusion:In human osteosarcoma, expressions of 14-3-3theta were increased and correlated with human osteosarcoma progression. 14-3-3theta may serve as a candidate biomarker in human osteosarcoma.

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