| 刘香吉 刘福国 宋文 王惠 江月萍.K-ras基因突变及表达与胰腺癌的关系[J].现代生物医学进展英文版,2015,15(26):5042-5046. |
| K-ras基因突变及表达与胰腺癌的关系 |
| Correlation of the Mutations and Expression of K-ras Gene with PancreaticCancer |
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| DOI： |
| 中文关键词: 胰腺癌 K-ras 基因 基因突变 诊断 |
| 英文关键词: Pancreatic neoplasms K-ras gene Gene mutation Diagnosis |
| 基金项目:青岛市科技局项目（11-2-3-1-(12)-nsh) |
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| 中文摘要: |
| 目的：探讨K-ras基因突变及蛋白表达对胰腺癌的诊断价值。方法：采用突变等位基因特异性扩增(PCR-MASA)检测31 例胰
腺癌和22 例非胰腺癌石蜡包埋组织中K-ras基因12 密码子点突变情况，并通过免疫组化法检测其K-ras蛋白的表达。结果：胰腺
癌患者K-ras基因12密码子点突变率为80.6 %(25/31)，K-ras 蛋白表达阳性率为87.1 %(27/31)；而非胰腺癌患者K-ras 基因12 密
码子点突变率为27.3 %(6/22)，K-ras 蛋白表达阳性率31.8 %(7/22)。胰腺癌患者K-ras 基因突变率及K-ras蛋白表达阳性率均明显
高于非胰腺癌患者(P 均＜0.05)。K-ras 基因突变与其蛋白表达呈正相关(r=0.542，P<0.05)，而与胰腺癌患者的性别、年龄、肿瘤部
位、肿瘤大小、临床分期及分化程度等临床病理特征均无显著相关性(P 均>0.05)。结论：胰腺癌K-ras 基因突变的发生率升高，且
K-ras蛋白表达异常上调，二者可能有助于胰腺癌的诊断。 |
| 英文摘要: |
| Objective:To investigate the clinical significances of K-ras gene mutation and K-ras protein expression for the diagnosis
and of pancreatic cancer.Methods:The mutation of K-ras gene 12 codon was detected by mutant allele-specific amplification
(PCR-MASA) and K-ras protein expression was detected by immunohistochemistry method in 31 cases of pancreatic cancer and 22 cases
of non-pancreatic cancer.Results:In 31 cases of pancreatic cancer, the mutation rate of K-ras gene 12 codon was 80.6%(25/31), and the
positive rate of K-ras protein expression was 87.1% (27/31). In 22 cases of non-pancreatic cancer, the mutation rate of K-ras gene 12
codon was 27.3%(6/22 ), and the positive rate of K-ras protein expression was 31.8%(7/22). The K-ras gene mutation rate and the positive
rate of K-ras protein expression of pancreatic cancer were obviously higher than the those of the non-pancreatic cancer (P＜0.05).
K-ras gene mutation and K-ras protein expression was positively correlated (r=0.542, P<0.05). K-ras gene mutation and K-ras protein expression
were not associated with the pathological characteristics of pancreatic cancer (gender, age, tumor location, tumor size, TNM
staging and degree of differentiation).Conclusion:The K-ras gene mutation rate and K-ras protein expression were upregulated in pancreatic
cancer, which may be helpful for the diagnosis of pancreatic cancer. |
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