| 张欣 向荣 李晓曦 林艳 刘珊珊 巩思嘉 潘金顺 赵子建△.膳食中高n-6/n-3多不饱和脂肪酸比值对小鼠肠道菌群的影响[J].现代生物医学进展英文版,2015,15(25):4824-4827. |
| 膳食中高n-6/n-3多不饱和脂肪酸比值对小鼠肠道菌群的影响 |
| High Ratio of Dietary n-6/n-3 Polyunsaturated Fatty Acids Affects GutMicrobiota in Mice |
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| DOI： |
| 中文关键词: 肠道菌群 多不饱和脂肪酸比值 花生四烯酸 |
| 英文关键词: Gut microbiota Polyunsaturated fatty acids ratio Arachidonic acid |
| 基金项目:国家重点基础研究发展计划973项目( 2013CB945200)；国家自然科学基金项目(81372798，81200570 )；
江苏省科技支撑计划(BE2012756 )；江苏省自然科学基金项目(BK20130059，BK2011766) |
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| 中文摘要: |
| 目的：探讨小鼠膳食中高n-6/n-3 多不饱和脂肪酸比值对肠道菌群的影响。方法：随机选取健康的30 只C57/B6 小鼠分为对
照组（基础饲料）、花生四烯酸组（基础饲料+10 %花生四烯酸）、鱼油组（基础饲料+10 %鱼油）。喂食16 周，16 周后提取小鼠肠道
菌群宏基因组DNA，采用Roche 454 测序技术对肠道菌群16SrRNA 基因V3-V5区域进行测序，对菌群的组成结构以及含量的变
化进行分析。结果：花生四烯酸组小鼠体内厚壁菌门的含量达到(55.3± 5.26) %，与对照组小鼠体内厚壁菌门的含量(30.23± 8.75)
%相比显著性升高(P<0.05)。并且花生四烯酸组小鼠体内变形菌门的含量(3± 0.762) %与对照组(1.5± 0.265) %相比也显著性上升
(P<0.05)。结论：膳食中高n-6/n-3 多不饱和脂肪酸比值会造成小鼠体内肠道菌群组成结构的失衡，导致厚壁菌门以及变形菌门的
含量上升。 |
| 英文摘要: |
| Objective:To observe the effects of dietary n-6/n-3 polyunsaturated fatty acids (PUFAs) on gut microbiota in mice.Methods:Thirty healthy C57/B6 male mice were randomized and divided into the control group (regular control diet), arachidonic acid
group (control diet +10% arachidonic acid), and fish oil group (control diet +10% fish oil). After 16 weeks of feeding, fecal bacterial
genomic DNA was extracted and PCR amplified based on the V3-V5 region of bacterial 16S rRNA gene for sequencing. Using such
information, the change of gut microbiota was evaluated.Results:The content of firmicutes in arachidonic acid group was (55.3± 5.26)% ,
which was significantly higher than that in the control group (30.23± 8.75)% (P<0.05). In addition, the content of Proteobacteria in the
control group (1.5± 0.265)%was lower than that in the arachidonic acid group (3± 0.762)%(P<0.05).Conclusion:High ratio of dietary
n-6/n-3 PUFAs causes the imbalance of gut mcriobiota in mice, particularly in firmicutes and proteobacteria. |
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