| 张敏 张涛 谢丽娟 曹萍 于成功.Faecalibacterium prausnitzii对大肠癌细胞增殖和凋亡的影响[J].现代生物医学进展英文版,2015,15(25):4801-4804. |
| Faecalibacterium prausnitzii对大肠癌细胞增殖和凋亡的影响 |
| Effect of F.prausnitzii on Proliferation and Apoptosisof Human Colorectal Tumor Cells |
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| DOI： |
| 中文关键词: F.prausnitaii 肿瘤细胞；增殖抑制；凋亡 |
| 英文关键词: F.prausnitaii Tumor Cells Proliferation Inhibition Apoptosis |
| 基金项目:国家自然科学基金项目（81470819） |
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| 中文摘要: |
| 目的：通过观察Faecalibacterium prausnitzii(F.prausnitaii )及其上清体外对大肠癌细胞增殖和凋亡的影响，探讨Faecalibacterium prausnitzii
的抗肿瘤作用。方法：将大肠癌LoVo 细胞培养于96 孔板，待细胞贴壁后加入不同浓度的F.prausnitaii(l× 107 /mL、1× 108 /mL、
1× 109 /mL)及其上清(1/2、1/5 浓度)，共同培养24 h或48 h后，以MTT 法检测F.prausnitaii及其上清对LoVo细胞的增殖抑制作
用；将大肠癌LoVo 细胞培养于6 孔板，待细胞贴壁后加入不同浓度的F.prausnitaii及其上清(同前)，共同培养48 h后用Annexin
V-FITC/PI双染色法对细胞染色后在流式细胞仪上检测F.prausnitaii及其上清对LoVo 细胞凋亡的影响。结果：MTT 结果显示，与
对照组相比，不同浓度的F.prausnitaii及其上清对大肠癌LoVo 细胞具有显著的增殖抑制作用(P<0.05)，且F.prausnitaii及其上清
浓度越大、作用时间越长，其抑制作用越明显(P<0.05)，具有浓度和时间依赖性。流式细胞结果显示，与对照组相比，不同浓度的F.prausnitaii
及其上清均具有诱导大肠癌LoVo 细胞凋亡的作用，且浓度越大，诱导作用越明显(P<0.05)，具有浓度依赖性。结论：
F.prausnitaii及其上清在体外具有抗肿瘤作用，其机制可能是通过诱导肿瘤细胞凋亡。 |
| 英文摘要: |
| Objective:The aims of current study were to investigate the antineoplastic activity of F.prausnitaii through observing
in vitro the effect of and its supernatant on proliferation and apoptosis of colorectal tumor cells.Methods:The LoVo cells
were first cultured in 96 well plates, then the LoVo cells were cultured for 24 h or 48 h with different concentrations of (l×
107/mL, 1× 108/mL and 1× 109/mL) or its supernatant (1/2 and 1/5 Concentration) respectively after cell attachment, then the inhibitory
effect on proliferation of LoVo cells was measured by MTT test. The LoVo cells were first cultured in 6 well plates, then the LoVo cells
were cultured for 48 h with different concentrations of F.prausnitaii and its supernatant (the same as before) respectively after cell
attachment, then the effect of and its supernatant on apoptosis of LoVo cells was measured by flow cytometry after staining
LoVo cells with Annexin V-FITC/PI.Results:Result F.prausnitaii of MTT showed and its supernatant with different concentrations
could inhibit proliferation of LoVo cells compared to control group (P<0.05), and more and more obvious inhibitory effect with higher
concentration or longer time showed time dependence and concentration dependence (P<0.05). Result of flow cytometry showed
or its supernatant with different concentrations could induce apoptosis of LoVo cells compared to control group (P<0.05), and
that effect increaseed with higher concentration showed concentration dependence (P<0.05).Conclusion:Both F.prausnitaii and its
supernatant have antineoplastic activity on tumor cells which are probably due to the induction of apoptosis. |
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