| 汪晓霞 王婵媛 林颖慧 武晓碧 张希尧.缬沙坦对糖尿病大鼠心肌保护作用以及氧化应激的影响[J].现代生物医学进展英文版,2015,15(24):4627-4630. |
| 缬沙坦对糖尿病大鼠心肌保护作用以及氧化应激的影响 |
| Effect of Valsartan on Myocardial Protection and Oxidative Stressin Diabetic Rats |
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| DOI： |
| 中文关键词: 糖尿病 心肌 氧化应激 缬沙坦 凋亡 |
| 英文关键词: Diabetic Myocardium Oxidative stress Valsartan Apoptosis |
| 基金项目:黑龙江省教育厅（12521196） |
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| 中文摘要: |
| 目的：探讨缬沙坦对糖尿病大鼠心肌的保护作用及氧化应激影响。方法：以链脲佐菌素建立糖尿病大鼠模型，缬沙坦干预治
疗12 周后，采用ELISA 法检测血清中8 脱氧鸟酐（8-OHdG）含量、超氧化物歧化酶（SOD）活性，PCR 测定心肌NADPH 氧化酶
亚型NOX2mRNA、p47phox mRNA表达，采用原位末端标记法（TUNEL）检测心肌细胞凋亡。结果：糖尿病大鼠经缬沙坦干预治疗
后，8-OHdG 含量，NOX2 和p47phox mRNA 表达均显著降低（P＜0.05），SOD 活性升高（P＜0.01），心肌细胞凋亡指数显著降低
（P＜0.05）。结论：高血糖导致糖尿病大鼠氧化应激增强和心肌细胞凋亡增加，缬沙坦可降低糖尿病大鼠氧化应激反应及减少心肌
细胞凋亡，因而对心肌有一定的保护作用。 |
| 英文摘要: |
| Objective:To explore the effects of Valsartan on cardiomyocyte apoptosis and oxidative stress in diabetic rats.Methods:Male mice received streptozotocin to induce diabetes. After 12 weeks of Valsartan treatment, The levels of serum8-OHdG and activity of
SOD were measured by ELISA, the NADPH oxidase subunits NOX2, and p47 phox mRNA expressions in rat myocardium tissues were
detected with real-time fluorescence quantitative polymerase chain reaction (PCR). Apoptosis was evaluated by means of terminal-deoxynucleotidyl
transferase mediated d-UTP nick end labeling (TUNEL).Results:Our results showed significant decrease in the expressions
of NOX2 and p47 phox mRNA (P<0.05), levels of 8-OHdG (P<0.01) and the apoptotic index, a marked increase in the activity of
SOD (P<0.01) in cardiac tissues of rats after treatment with Valsartan.Conclusion:High glucose can induce oxidative stress and cardiomyocyte
apoptosis, while Valsartan may inhibit oxidative stress and cardiomyocyte apoptosis, thereby plays a protective role in the
cardiomypathy of diabetic rats. |
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