| 刘华 付文静 邓英辉 林娜 李文 董星彤 贾强.氧化低密度脂蛋白促进培养的人肾小球系膜细胞LOX-1 表达[J].现代生物医学进展英文版,2015,15(23):4435-4439. |
| 氧化低密度脂蛋白促进培养的人肾小球系膜细胞LOX-1 表达 |
| In vitro Study of LOX-1 Expression on Human Mesangial Cell Induced byOxidized LDL |
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| DOI： |
| 中文关键词: 氧化低密度脂蛋白 人肾小球系膜细胞 植物血凝素样氧化低密度脂蛋白受体 |
| 英文关键词: Ox-LDL Human mesangial cell Lectin like Ox-LDL receptor 1 |
| 基金项目:北京市科委资助项目（SCW2009-07） |
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| 中文摘要: |
| 目的：研究氧化低密度脂蛋白（Ox-LDL）对人肾小球系膜细胞植物血凝素样受体（lectin-like oxidized low-density lipoprotein
receptor，LOX-1）表达。方法：不同浓度的Ox-LDL和培养的人肾小球系膜细胞共孵育，应用Real-time PCR和Western Blot 方法检
测Ox-LDL对人肾小球系膜细胞LOX-1 表达的影响。结果：Ox-LDL剂量和时间依赖性促进人肾小球系膜细胞LOX-1mRNA和
蛋白表达。Ox-LDL 40 ug/mL刺激细胞0-24 小时，于12 小时达峰值。Ox-LDL 10、20、40、60 ug/mL 分别作用于细胞12 小时，40
ug/mL组达到峰值，为基础值的3.73 倍。Ox-LDL 40 ug/mL刺激细胞0-24 小时，LOX-1 蛋白24 小时达高峰，Ox-LDL 10、20、40、
60 ug/mL分别作用细胞24 小时，40 ug/mL组细胞LOX-1 蛋白达到峰值，为基础值的1.81 倍。结论：Ox-LDL 在一定浓度范围内
剂量和时间依赖性促进人肾小球系膜细胞LOX-1 表达。 |
| 英文摘要: |
| Objective:To observe whether the expression of lectin-like oxidized LDL receptor can be induced by Ox-LDL on
human mesangial cell (HMC).Methods:When cultured HMC stimulated by different doses of Ox-LDL, the levels of LOX-1 on HMC
induced by Ox-LDL were examined using Real-time PCR and Western Blot.Results:Ox-LDL could induce LOX-1 mRNA and protein
expression in a dose-dependent manner (10-40 ug/mL). The peak level of LOX-1 mRNA reached after 12 h of stimulation and was as
high as 3.73-fold as control. The up-regulation effect on LOX-1 protein reached peak after 24h and was as high as 1.81-fold as control
treated with 40 ug/ml of Ox-LDL.Conclusion:Ox-LDL can induce LOX-1 expression in a dose and time dependent manner. |
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