| 智慧兰 张健 李龙虎 金哲 刘天卿 王美兰 于勤.PDE5 shRNA对小鼠急性心梗后早期细胞凋亡的影响[J].现代生物医学进展英文版,2015,15(23):4427-4430. |
| PDE5 shRNA对小鼠急性心梗后早期细胞凋亡的影响 |
| Adenoviral Short Hairpin RNA Targeting Phosphodiesterase 5 InhibitsMyocardial Cells Apoptosis at Early Stage Post-MI |
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| DOI： |
| 中文关键词: 短发夹RNA 干预治疗 心肌梗死 磷酸二酯酶5 抑制剂 |
| 英文关键词: Short hairpin RNA Interference therapy Myocardial infarction Phosphodiesterase 5 |
| 基金项目:国家自然科学基金项目(81170187) |
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| 中文摘要: |
| 目的：探讨磷酸二酯酶5（PDE5）shRNA对小鼠急性心梗后早期细胞凋亡的影响。方法：通过结扎小鼠左冠状动脉前降支建
立小鼠心肌梗死模型，并将建立好的模型随机分为实验组(n=10)：将携带有抑制PDE5 的shRNA（PDE5 shRNA）重组腺病毒载体
对小鼠心肌组织多部位注射；模型组(n =10)：将没有插入PDE5 shRNA的普通腺病毒载体对小鼠心肌组织多部位注射。1 周后，进
行免疫组化和TUNEL 分析检测梗死及梗死周边细胞凋亡情况，ELISA 法检测环磷酸鸟苷（cyclic guanosine monophosphate，
cGMP）和蛋白激酶G（protein kinase G,PKG）的表达，免疫蛋白印迹分析检测各组磷酸二酯酶5（phosphodiesterase5,PDE5）的表达
情况。结果：心梗1 周后，和模型组相比较，实验组小鼠梗死区及梗死周边区域细胞凋亡明显减少（P<0.05），实验组小鼠心肌
PDE5表达明显减少，cGMP 和PKG 表达明显上调（P<0.05）。结论：PDE5 shRNA 可以明显减少梗死区及梗死周边区细胞凋亡，可
能与上调cGMP 和PKG 的表达密切相关。 |
| 英文摘要: |
| Objective:To study the impact of PDE5 shRNA on myocardial cells apoptosis at early stage post-MI.Methods:Myocardial infarction (MI) was induced in mice by left coronary artery ligation. Mice were randomly assigned to test group（n=10）, in
which adenovirus vector with PDE5 shRNA particles were injected along the anterior and posterior LV wall, and model group（n=10）, in
which adenovirus vector without PDE5 shRNA particles were injected in the same manner. One week post-MI, apoptosis was evaluated
using the TUNEL method, Proteins were extracted from the left ventricular of heart, level of PDE5 expression was detected using
Western Bloting, and level of cGMP or PKG activity in the left ventricular of heart was evaluated using ELISA.Results:One week
post-MI, compared to model group, test group had reduced number of apoptotic cells in infarct and periinfarct areas, and significantly
reduced cardiomyocytes apoptosis in the peri-infarct regions (P<0.05). Compared to model group, the level of PDE5 significantly
decreased and the levels of cGMP and PKG significantly increased in the test group (P<0.05).Conclusion:The present study suggests
the protective effects of PDE5 shRNA on acute myocardial infarction, significantly inhibiting myocardial cell apoptosis through possible
activating cGMP/PKG signaling pathway. |
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