| 林龙龙 杨兆娟 钱钰 夏苏华 张力 李静怡 马爱辉 许东旭 王博石 刘昀 刘永忠.NDRG1 对人肠癌细胞失巢凋亡的影响[J].现代生物医学进展英文版,2015,15(23):4406-4409. |
| NDRG1 对人肠癌细胞失巢凋亡的影响 |
| Effect of NDRG1 on the Anoikis in Human Colon Cancer Cells |
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| DOI： |
| 中文关键词: NDRG1 失巢凋亡 肠癌 |
| 英文关键词: NDRG1 Anoikis Colon cancer |
| 基金项目:国家自然科学基金项目(81201542) |
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| 中文摘要: |
| 目的：探讨NDRG1 对体外培养的人肠癌细胞系失巣凋亡的影响。方法：采用慢病毒系统将NDRG1 表达单元转入人肠癌细
胞系SW620、HCT8 中，建立相应的过表达稳定细胞系；通过siRNA 的方法干扰HCT116 和LOVO细胞系中NDRG1 的表达，分
别在非贴壁培养的情况下培养48 小时，采用流式细胞术和TUNEL染色检测细胞的凋亡情况。结果：在贴壁培养条件下，NDRG1
过表达并没有显著影响肠癌细胞的生长及增殖，而NDRG1 特异性siRNA 干扰HCT116 细胞中NDRG1的表达后，其凋亡率无明
显变化(P>0.05)。在悬浮培养条件下，NDRG1 过表达的肠癌细胞的失巢凋亡率显著低于正常对照组(P<0.05)，而用三种不同的
siRNA干扰HCT116 及LOVO细胞中NDRG1 的表达后，其失巢凋亡率均显著高于正常对照组(P<0.05)。结论：NDRG1 在体外可
抑制人肠癌细胞的失巢凋亡。 |
| 英文摘要: |
| Objective:To explore the effects of NDRG1 on anoikis of human colon cancer cells.Methods:NDRG1 gene was
lentivirally tranduced in the human colon cancer cells (SW620 and HCT8) to establishment stable cell lines, and siRNA was used to
decrease the NDRG1 expression in HCT116 and LOVO cell lines. Flow cytometry and TUNEL staining were used to detect the apoptosis
in cells treated under suspension condition for 48 h.Results:Under the adherent culture condition, overexpression of NDRG1 had no
effects on the growth and proliferation of human colon cancer cells, while the decrease of NDRG1 expression with specific siRNA had
no effect on the apoptosis in HCT116 cells(P>0.05). Under the suspension culture condition, the anoikis rate of human colon cancer cells
overexpressed NDRG1 was significantly lower than that of the control group (P<0.05), while the anoikis rates of HCT116 and LOVO
cells treated by three kinds of NDRG1 siRNA were both obviously higher than those of the control group(P<0.05).Conclusion:NDRG1
functioned in maintaining cell survival of cultured colon cancer cells, mainly manifesting the enhancement of the anoikis-resistance. |
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