| 张昊 印海翔 徐颺 刘涛生.MK-801 诱导精神分裂症小鼠的c-Fos及NADPH氧化酶通路机制研究[J].现代生物医学进展英文版,2015,15(22):4252-4256. |
| MK-801 诱导精神分裂症小鼠的c-Fos及NADPH氧化酶通路机制研究 |
| Involvement of c-Fos and NADPH Oxidase Pathway in the Pathogenesis ofSchizophrenia in Mice Induced byMK-801 |
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| DOI： |
| 中文关键词: 精神分裂症 MK-801 c-Fos 氧化应激 |
| 英文关键词: Schizophrenia MK-801 c-Fos Oxidative stress |
| 基金项目:国家自然科学基金青年项目（31200844） |
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| 中文摘要: |
| 目的：探讨MK-801 诱导的精神分裂症小鼠的相关机制，为相关精神分裂症的临床研究和治疗提供参考。方法：60只昆明小
鼠随机分为3 组：对照组、低剂量模型组和高剂量模型组（n=20）。采用两个剂量NMDA受体拮抗剂MK-801 建立谷氨酸低下精
神分裂症小鼠模型，Western Blot 分析小鼠海马组织中相关蛋白c-Fos 及NADPH 氧化酶亚基（p22、p47、p67）的表达变化。结果：
低剂量MK-801 能够显著增加模型组小鼠的自发活动及刻板行为，且与对照组相比有显著的统计学差异性（P<0.01），c-Fos 蛋白
及NADPH 氧化酶蛋白亚基p22 和p47 的表达均明显高于对照组（P<0.01）。而高剂量的MK-801 对小鼠具有后肢肌力障碍方面
的毒性作用。结论：低剂量的MIK-801 能诱导小鼠精神分裂症模型，且氧化应激及c-Fos的过表达参与了MK-801诱导的小鼠精
神分裂症的发病过程。 |
| 英文摘要: |
| Objective:To explore the pathogenesis mechanism of schizophrenia induced by MK-801 in order to provide reference
for clinical research and treatment.Methods:Mice were divided into 3 groups, control group, low dose group and high dose group
(n=20). The schizophrenia mice model was established by injecting MK-801, and the expression of c-Fos and NADPH oxidase units was
detected by Western blot.Results:The activity distance and stereotyped behavior were enhanced in the low dose and high dose of
MK-801 group (P<0.01), and the expression of c-fos, and the NADPH oxidase units, p22 and p47 were upregulated greatly compared
with control group (P<0.01). But high dose of MK-801 indicated toxic role for myodynamia disorder of legs.Conclusion:c-Fos
overexpression and oxidative stress participated in the pathogenesis of schizophrenia in mice induced by MK-801. |
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