| 蒋巍亮 曾悦 卢战军 肖军华 郑萍 万荣 王兴鹏 杨丽娟.SHIP在炎症性肠病结肠组织中的表达及其临床意义[J].现代生物医学进展英文版,2015,15(21):4045-4048. |
| SHIP在炎症性肠病结肠组织中的表达及其临床意义 |
| Expression and Its Significance of SHIP in Intestinal Mucosa of InflammatoryBowel Disease |
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| DOI： |
| 中文关键词: 炎症性肠病 溃疡性结肠炎 克罗恩病 SHIP (含SH2 结构域的肌醇5' 磷酸酶) |
| 英文关键词: Inflammatory bowel disease(IBD) Ulcerative colitis(UC) Crohn's disease(CD) SHIP (Src homology 2 domain-containing
inositol 5'-phosphatase) |
| 基金项目:卫生部行业科研项目（201002020）；国家自然科学基金青年项目（81200321）；
上海市科委项目（09DZ1906903）；上海交通大学医工交叉重点项目（YG2014ZD10） |
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| 中文摘要: |
| 目的：观察SHIP 在溃疡性结肠炎(UC)和克罗恩病(CD)患者结肠组织标本中的表达情况，探讨其在炎症性肠病发生过程中
所起的作用及意义。方法：收集活动期UC患者，活动期CD 患者，及结直肠癌旁正常粘膜组织（NC组）标本各20 例。将活检标本
进行苏木精- 伊红染色及SHIP 免疫组化染色观察；利用Western blot 半定量比较分析SHIP 蛋白表达及组间差异；Real-time
RT-PCR 分析SHIP在RNA水平的表达情况和组间差异。统计学处理采用Student's t检验。结果：免疫组化染色示UC组SHIP阳
性表达积分为（7.20± 2.53），CD 组积分为（6.50± 2.76），对照组积分为（1.10± 0.74）。t 检验组间比较UC组和CD组无统计学差异
(t=0.59，P＞0.05)；而UC组与NC组（t=7.32，P＜0.05），CD组与NC组（t=5.98, P＜0.05），差异均有统计学意义。Western blot 检测
结肠组织SHIP表达，UC组SHIP 相对表达量为（0.314± 0.021），CD组（0.301± 0.019），NC 组（0.163± 0.027）。UC和CD组表达
无差异(t=1.44，P＞0.05)，而UC组，CD组与NC 组相比表达明显升高（t=13.88、13.16, P均＜0.05）。Real-time RT PCR 检测UC 组
结肠粘膜SHIP mRNA相对表达量为（0.649± 0.028），CD 组为（0.645± 0.021），NC 组为（0.140± 0.015）。同样，UC组与CD 组没
有统计学差异，而其相较对照组表达均升高（P＜0.05）。结论：炎症性肠病患者结肠组织SHIP 表达明显高于正常结肠组织，但其在
溃疡性结肠炎和克罗恩病间没有明显差异；提示SHIP可能在炎症性肠病的发病中发挥重要作用。 |
| 英文摘要: |
| Objective:To investigate the expresssion of SHIP in the intestinal mucosa of patients with ulcerative colitis (UC) or
Crohn's disease(CD), and explore its role and relevance in the pathogenesis of inflammatory bowel disease(IBD).Methods:The intestinal
or colonic mucosa biopsy specimens were collected from 20 active UC patients and 20 active CD patients had enteroscopy examination,
and 20 patients underwent colorectal surgery. The colon tissues were taken for hematoxylin-eosin staining and immunohistochemieal
staining (SHIP) observation. Western blot was performed for detection of protein expression of SHIP in each group and qRT-PCR was
used to detect SHIP expression at RNA level. The data were analyzed by Student's t test.Results:The integral of SHIP positive expression
by immunohistochemical staining was (7.20± 2.53) in UC group, (6.50± 2.76) in CD group, and (1.10± 0.74) in negative control
group. There was no statistical significance between UC and CD groups (t=0.59,P＞0.05), but the differences between UC and NC（t=7.
32, P＜0.05）, CD and NC（t=5.98, P＜0.05）were statistically significant. The expression of SHIP in intestinal mucosa detected by western
blot was（0.314± 0.021）, （0.301± 0.019）, （0.163± 0.027）respectively in UC, CD, NC groups. No difference was found between
UC and CD(t=1.44, P＞0.05), but they were significantly higher than negative control（t=13.88, 13.16, P＜0.05). The expression of SHIP
mRNA analyzed by Real-time RT-PCR of three groups was（0.649± 0.028）,（0.645± 0.021）,（0.140± 0.015）in UC, CD, NC groups,
respectively and the results of statistical analysis was the same as above.Conclusion:The expression of SHIP in intestinal mucosa of
IBD patients was significantly higher than normal colonic tissue, but there was no difference between UC and CD patients; and it was
supposed that SHIP was closely correlated with the pathogenesis of inflammatory bowel disease. |
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