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目的：检测软骨细胞在不同浓度尿酸（uric acid, UA）环境下的活性及其白介素-1beta（IL-1beta）和肿瘤坏死因子-alpha（TNF-alpha）的表达水平的变化，探讨不同浓度的尿酸对软骨的影响。方法：分别用0、4、8、10、16、32 mg/dL的尿酸溶液培养大鼠关节软骨细胞， MTT 比色法检测软骨细胞活力，ELISA 方法检测细胞培养液中IL-1beta、TNF-alpha的浓度。结果：4 mg/dL UA 组软骨细胞活性低于对照组（0 mg/dL)，差异无统计学意义（P＞0.05)；8、10、16、32 mg/dL UA 组软骨细胞活性均低于对照组，差异有统计学意义（P＜ 0.05)，且其活性随着尿酸浓度的升高而降低。4、8、10 mg/dL UA 组IL-1beta、TNF-alpha的水平均高于对照组，差异有统计学意义（P＜ 0.05)，其水平随尿酸浓度的升高而增加。10、16、32 mg/dL UA 组IL-1beta、TNF-alpha的水平亦均高于对照组，差异有统计学意义（P＜ 0.05)，但其水平随着尿酸浓度的升高而降低。结论：尿酸可以抑制软骨细胞的活性；同时，在4-10 mg/dL浓度范围内明显促进软骨细胞IL-1beta、TNF-alpha的表达，但高于10 mg/dL后，随着细胞活性的严重降低其促进作用逐渐减弱。这可能是尿酸参与软骨破坏及骨关节炎发生的重要因素。

英文摘要:

Objective:To detect the cell viability and the expression of interleukin-1 beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha) of chondrocytes which were treated with different concentrations of uric acid (UA) in vitro, to explore the influence of different concentration of uric acid on the cartilage.Methods:The articular cartilage cells of rats were cultured in vitro, and then the cells were treated with uric acid solution of the concentrations of 0, 4, 8, 10, 16, 32 mg/dL for 24 hours respectively. The cell viability of rat articular cartilage was assessed by MTT. The levels of IL-1beta and TNF-alpha in cell supernatant fluid were measured by ELISA.Results:The cell viability of 4 mg/dL UA group was lower than that in the control group (0 mg/dL), having no difference (P＞0.05); The cell viability of 8, 10, 16, 32 mg/dL UA groups was significantly lower than that in the control group and decreased with the increase of the concentration of uric acid (P＜0.05). The levers of IL-1beta and TNF-alpha in 4, 8, 10 mg/dL UA groups were higher than that in the control group (P＜0.05) and increased with the increase of the concentration of uric acid. The levers of IL-1beta and TNF-alpha in 10, 16, 32 mg/dL UA groups were higher than that in the control group (P＜0.05) but decreased with the increase of the concentration of uric acid.Conclusion:Uric acid inhibits cell viability of cartilage cells; at the same time, it significantly promotes the expression of IL-1beta and TNF-alpha in the range of 4-10 mg/dL. However, after more than 10 mg/dL of uric acid, this effect is gradually weakened with the serious decline of cell viability. This may be the important factors that uric acid involves in the cartilage damage, as well as the pathogenesis of osteoarthritis.

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