| 于丹 伍志强 梅倩 白桦 韩为东 孟元光.LRP16 影响宫颈癌Siha细胞对化疗药物的敏感性[J].现代生物医学进展英文版,2015,15(20):3850-3854. |
| LRP16 影响宫颈癌Siha细胞对化疗药物的敏感性 |
| Significance of Chemotherapy Sensitivity of the Expression Level ofthe Human LRP16 Gene in Siha Cells |
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| DOI： |
| 中文关键词: LRP16 Siha 细胞增殖 细胞凋亡 细胞周期 |
| 英文关键词: LRP16 Siha Cell proliferation Cell apoptosis Cell cycle |
| 基金项目:国家自然科学基金项目(81071617，81001184) |
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| 中文摘要: |
| 目的：探讨抑制LRP16 的表达对宫颈癌Siha 细胞的化疗药物敏感性的影响。方法：将抑制LRP16 表达的小干扰RNA:negativecontrol-
siRNA(NC)、siRNA-374(si374)转染入Siha 宫颈鳞癌细胞系中，通过顺铂（DDP）和紫杉醇(TAX)的处理后，采用CCK-8
检测不同浓度紫杉醇、顺铂作用宫颈癌细胞系Siha48 h 后，计算出细胞被抑制一半时顺铂、紫杉醇的药物浓度(IC50) ；使用
Hoechst33342 染色观察细胞凋亡，采用流式细胞仪检测顺铂IC50 作用Siha细胞48 小时后的细胞凋亡情况，紫杉醇IC50 作用
Siha 细胞之后的细胞周期分布情况。结果：CCK-8 检测转染的Siha 细胞增殖活性受到抑制，Hoechst33342 染色观察转染的Siha
细胞凋亡明显增加，流式细胞仪检测凋亡显示，si374+顺铂的早期凋亡率22.15± 2.24，NC+顺铂12.45± 2.72，流式细胞仪检测周
期显示G2/M（%），si374+紫杉醇29.94± 1.87，NC+紫杉醇17.66± 2.32。结论：LRP16 基因表达下调之后，抑制Siha 细胞的增殖、
促进其凋亡，使细胞周期滞留于G2/M期，从而提高Siha 细胞的化疗敏感性。 |
| 英文摘要: |
| Objective:To investigate the effect of the inhibition of expression of LRP16 in Siha cervical cancer cells on
chemotherapeutic drug sensitivity.Methods:Cervical squamous carcinoma cell (Siha cell) was transfected with small interfering RNA
which was as negative control[siRNA(NC)] and small interfering RNA which was inhibited the expression of LRP16[siRNA-374(si374)],
then was treated with cisplatin and paclitaxel. Siha cell was detected by CCK-8 with the treatment of different concentrations of cisplatin
and paclitaxel after 48 hours. The drug (cisplatin or paclitaxel) concentration was calculated when Siha cells were half inhibited; Cell
morphology was observated by Hoechst33342 staining. Flow cytometry was utilized to detect the apoptosis of Siha cells after the
treatment of cisplatin IC50 48 hours. G2/M distributionof the cell cycle was detected by flow cytometry after the treatment of paclitaxel
IC50 48 hours.Results:The proliferative activity of transfected Siha was inhibited by CCK-8 detection and the apoptosis of transfected
Siha was increased significantly when detectedby Hoechst33342.The early apoptosis rate of siLRP16+cisplatin was 22.15± 2.24, while the
rate of NC+ cisplatin was 12.45± 2.72 by flow cytometry. The G2/Mdistribution rate of siLRP16+paclitaxel was 29.94± 1.87, while NC+
paclitaxel was17.66± 2.32.Conclusion:After the down-regulated expression of LRP16 gene,the proliferation of Siha cells was inhibited,
the apoptosis of Siha was induced and the cell cycle remained in the G2/Mphase, thereby enhancing chemosensitivity of Siha cells. |
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