| 申福国 孔维丽 吕涵宁 武广海 崔宏宇 刘士臣 赵鹏 郭全义 杨建华.自体软骨细胞修复兔膝关节全层软骨缺损的实验研究[J].现代生物医学进展英文版,2015,15(20):3839-3842. |
| 自体软骨细胞修复兔膝关节全层软骨缺损的实验研究 |
| Experimental Study of Autologous Chondrocyte Repairof Rabbit Full-thickness Cartilage Defects of the Knee |
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| DOI： |
| 中文关键词: 人脐带Wharton 胶 支架 软骨细胞 软骨缺损 |
| 英文关键词: Human umbilical cord Wharton's jelly matrix Scaffold Chondrocytes Cartilage defects |
| 基金项目:佳木斯大学研究生科技创新项目（LZR2014_022）；国家级大学生创新创业训练项目(1110150221) |
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| 中文摘要: |
| 目的：研究自体软骨细胞复合于人脐带Wharton胶支架对兔膝关节全层软骨缺损的修复效果。方法：经自体关节软骨细胞
经体外培养后复合到制备人脐带Wharton 胶取向支架内构建细胞- 支架复合体，选取健康清洁新西兰兔23 只，雌雄不拘，体重
2.5-3.0 kg，取滑车沟中下部制作全层软骨缺损模型后随机分成A、B和C 组。A组(n= 10)：植入自体软骨细胞+人脐带Wharton
胶取向支架复合体；B组(n= 10)：植入单纯人脐带Wharton 胶取向支架；C组(n= 3)：不做任何处理正常兔。分别于术后3 个月和6
个月各处死后取材进行生物力学特性评估检测。结果：压痕实验显示在3 个月时A 和B 组修复区组织刚度分别达到正常软骨的
45.72%和25.25%，且A组刚度明显优于B组，均低于C组( P<0.05)；到6 个月时各自达到正常软骨刚度的69.76%和35.14%，同
样A 组刚度明显优于B 组，均低于C 组( P<0.05)且在同期个各组之间均有显著性差异（F=80.309，P<0.05）。结论：体外培养的自
体软骨细胞与人脐带Wharton 胶复合在体内的微环境作用下修复软骨缺损效果良好，为软骨组织工程提供了一种新支架材料。 |
| 英文摘要: |
| Objective:To investigate the effect of autologous chondrocytes in human umbilical cord Wharton's jelly matrix
scaffold stents on rabbit knee full thickness cartilage defect repair.Methods:Autologous chondrocyte after in vitro preparation of the
composite to build cells from human umbilical cord Wharton's jelly matrix glue oriented scaffolds - stents complex, choose healthy and
clean 23 rabbits, male or female, weighing 2.5-3.0 kg, take the lower pulley groove were randomly divided into A, B and C after making
a full thickness cartilage defect model. GroupA (n=10): autologous chondrocyte implantation+human umbilical cord Wharton's jelly
matrix plastic composite scaffold orientation; groupB (n=10): implanted human umbilical cord Wharton's jelly matrix simple plastic
bracket orientation; group C (n=3): no process for normal rabbit. Were everywhere after 3 months and 6 months after the death of
biomechanical properties derived assessment test.Results:The indentation experiments are displayed in three months organizing stiffness
restoration area A and B , respectively 45.72%and 25.25%of normal cartilage and compare with group A, the stiffness was significantly
better than in group B, and both were lower than in groupC (P<0.05); to 6 months when each reached 35.14% and 69.76% of normal
cartilage stiffness , compare with group A, the stiffness was significantly better than in group B, and both were lower than in group C (P
<0.05) and in the same period of months between the groups was significant difference (F=80.309, P<0.05).Conclusion:Autologous
cultured chondrocytes and human umbilical cord Wharton's jelly matrix scaffold composite repair cartilage defects to good effect in vivo
microenvironment for cartilage tissue engineering provides a new scaffold. |
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