| 郭忠尚 毕龙 杨旻 燕明 张振宇 韩一生.万古霉素/ 磷酸钙骨水泥复合材料的细胞毒性研究[J].现代生物医学进展英文版,2015,15(20):3813-3816. |
| 万古霉素/ 磷酸钙骨水泥复合材料的细胞毒性研究 |
| Study on Cytotoxicity of Vancomycin/CalciumPhosphateCement Composite Materials |
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| DOI： |
| 中文关键词: 万古霉素 磷酸钙 细胞毒性 感染 |
| 英文关键词: Vancomycin Calciumphosphate cement Cytotoxicity Infection |
| 基金项目:国家自然科学基金项目（30901532，81371982） |
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| 中文摘要: |
| 目的：研究不同浓度配比（0%、1%、5%、10%）万古霉素/ 磷酸钙复合材料对大鼠骨髓间充质干细胞的毒性作用。方法：原代
培养大鼠间充质干细胞并鉴定；采用CCK-8 法测定不同浓度配比万古霉素/ 磷酸钙复合材料对大鼠骨髓间充质干细胞增殖的影
响、TUNEL法测定细胞凋亡率、扫描电镜观察细胞形态学改变。结果：CCK-8结果显示，5%及10%万古霉素/ 磷酸钙复合材料显
著抑制大鼠骨髓间充质干细胞增殖（P<0.05）；TUNEL 结果显示，5%及10%万古霉素/ 磷酸钙复合材料组细胞凋亡率显著增高
（P<0.05）；扫描电镜结果显示，高浓度万古霉素毒性作用下细胞失活，形态学发生显著变化；1%万古霉素/ 磷酸钙复合材料组对细
胞影响相对于空白对照组无显著差异。结论：低浓度（1%）万古霉素/ 磷酸钙复合材料基本无细胞毒性，细胞相容性好。 |
| 英文摘要: |
| Objective:To investigate the cytotoxicity of vancomycin (Van)/calcium phosphate cement (CPC) composite materials
on rat bone marrow mesenchymal stem cells (BMSCs).Methods:Primary BMSCs were isolated, cultured and identified. The cells were
incubated with different concentrations (containing 0%, 1%, 5%, 10% Van) of Van/CPC composite materials. The cell viability of
BMSCs was measured by CCK-8 method, and the cell apoptosis was detected by TUNEL method and the cell morphology was
observed-by scanning electron microscope (SEM).Results:5% and 10% Van/CPC composite materials could significantly inhibit the
proliferation of BMSCs (P<0.05), and increase apoptosis (P<0.05) and lead to higher cytotoxicity.1%Van/CPC exerted no obvious effect
on the BMSCs compared to the blank control.Conclusion:Low concentration of Van/CPC had no cytotoxicity on rat and BMSCs
performed good biocompatibility. |
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