| 郭琼 李卉 沙亚哈提·别尔克哈之 谌宏鸣 李惠武.新疆食管癌中Myc、TP53表达及其与临床病理因素相关性研究[J].现代生物医学进展英文版,2015,15(19):3637-3640. |
| 新疆食管癌中Myc、TP53表达及其与临床病理因素相关性研究 |
| Correlative Research between the Expression of Myc, TP53and Clinical Pathological Factors in EsophagealCancer of Xinjiang Area |
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| DOI： |
| 中文关键词: 食管癌 Myc TP53 新疆地区 |
| 英文关键词: Esophageal cancer Myc Tp53 Xinjiang area |
| 基金项目:国家自然科学基金项目（81350021） |
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| 中文摘要: |
| 目的：检测Myc和TP53在食管癌组织和远端无癌组织的表达情况，并分析其与临床病理因素之间的关系，初步探讨
Myc和TP53 在新疆食管癌发生发展中可能存在的特点。方法：经Trizol 一步法提取88 例新疆地区食管癌组织及其远端无癌组织标
本总RNA，mRNA逆转录为cDNA，经聚合酶链式反应生成产物，运用光密度值即半定量RT-PCR 技术检测88 例新疆地区食管
癌组织、远端无癌组织中Myc和TP53的mRNA 表达情况及二者阳性表达率，并分析和的表达与临床病理因素之间
的相关性。结果：①Myc的mRNA相对表达量在食管癌组织中高于远端无癌组织，差异有显著性（P＜ 0.01）； TP53的mRNA相对
表达量在远端无癌组织中高于癌组织（P＜0.05）；② Myc的阳性表达率在88 例食管癌组织中高于远端无癌组织（P＜0.05）；
TP53的阳性表达率在远端无癌组织中高于癌组织（P＜0.05）；③ Myc的表达与分化程度（P＜ 0.01）、TNM 分期（P＜0.01）、淋巴结转移
（P＜0.05）、侵犯深度（P＜0.05）和族别（P＜ 0.05）有关，与性别无关； TP53的表达与侵犯程度（P＜0.01）有关，与性别、分化程度、
TNM分期、淋巴结转移和族别均无关；④ 88 例食管癌组织中， Myc和TP53的表达呈现负相关（r = -0.501，P＜0.0 1）。结论：
Myc在新疆地区食管癌组织中表达上调， TP53则表达减弱。说明Myc参与食管癌的发生和发展，而则TP53可能保护正常组织不发生癌变。 |
| 英文摘要: |
| Objective:To test the expression of Myc and TP53 in 88 cases esophageal cancer and distal normal tissue in Xinjiang
area, and analyse the characteristic and clinical correlationship between and , further study the relationship of and in
the development of Xinjiang esophageal cancer.Methods:Trizol one step method was used to extract total mRNA from all cases, then
the mRNA was converted into cDNA by reverse transcriptase (the polymerase chain reaction). Applying RT-PCR half quantitative and
optical density value, the mRNA expression and the positive expression rate of Myc and TP 53was detected in 88 cases esophageal
cancer and distal normal tissue in Xinjiang area then the correlation between the expression of Myc, TP53 and clinical pathological
factors in esophageal cancer was analyzed.Results:① The relative value of Myc mRNA in cancer tissue was higher than in distal normal
tissue(P<0.01), while the ralative value of TP53 mRNA in distal normal tissue was higher than in cancer tissue(P<0.05); ② The positive
expression rate of Myc in cancer tissue was higher than in distal normal tissue (P<0.05),while the positive expression rate of TP53 was
opposite(P<0.05); ③ The positive expression of Myc was associated with differentiation degree(P<0.01), clinical stages(P<0.01), lymph
node metastasis (P<0.05), tumor invasion depth(P<0.05) and race(P<0.05);TP53 's positive expression was correlated with invasion depth
(P<0.01), and was uncorrelated with differentiation degree, clinical stages, lymph node metastasis, human race and sex; ④ In 88 cancer
cases, Myc expression was negative related to TP53(r = -0.501, P<0.01).Conclusion:Myc expression was increased in esophageal cancer
of Xinjiang area, and TP53 expression was decreased, which indicate Myc may play a certain role in the development of esophageal
cancer, but TP53 could prevent normal tissue to change into tumor. |
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