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目的：探讨无翅型MMTV整合位点家族成员10a（Wnt10a）在甲状腺乳头状癌(papillary thyroid carcinoma，PTC)和桥本合并甲状腺乳头癌（Hashimoto's thyroiditis associated with papillary thyroid carcinoma）中的表达及其临床意义。方法：采用qRT-PCR方法检测Wnt10a mRNA 在41 例新鲜甲状腺组织（PTC 组织18 例，HT 合并PTC 组织12 例，结节性甲状腺肿组织11 例）中的表达；用免疫组化SP法检测Wnt10a 蛋白在50 例甲状腺石蜡标本（PTC 组织20 例，HT合并PTC组织20 例，结节性甲状腺肿组织 10 例）中的表达。结果：① RT-PCR Wnt10a mRNA 在甲状腺癌组织中的表达明显高于其在良性甲状腺肿瘤组织中的表达(P < 0.01) ，差异有显著性；Wnt10a mRNA在PTC、HT合并PTC组织中的阳性表达强度分别为2.49× 10-5（0.68× 10-5，15.28× 10-5）， 1.26× 10-（5 0.97× 10-5，13.73× 10-5）；P<0.05，差异具有显著性。Wnt10a mRNA 在PTC、HT 合并 PTC 组织中的表达分别是结节性甲状腺肿组织的9.98、2.69 倍。②SP 法：Wnt10a 在甲状腺癌组织中的表达明显高于其在良性甲状腺肿瘤组织中的表达。Wnt10a mRNA 和蛋白在甲状腺癌组织中的表达与肿瘤大小、临床分期、淋巴结转移等临床因素无明显关系(P>0.05)，只与甲状腺癌组织的病理分型相关，（P<0.05)。结论：Wnt10a 在甲状腺乳头状癌和桥本合并甲状腺乳头癌中呈现高表达，且有差异性，有望作为甲状腺癌的病理分型的参考指标，有助于甲状腺癌的早期诊断及病理分型的判定，使患者得到及时有效治疗。

英文摘要:

Objective:To investigate the expression and clinical significance of Wnt10a in papillary thyroid carcinoma (PTC) and Hashimoto's thyroiditis associated with papillary thyroid carcinoma (HT-PTC).Methods:The relative expression of Wnt10a mRNA was determined by real time-polymerase chain reaction (RT-PCR) in 41 fresh thyroid tissues (18 PTC tissues, 12 HT with PTC tissues and 11 nodular goiter tissues). The relative expression of Wnt10a protein was determined by immunohistochemical SP method in 50 paraffinembedded thyroid tissues (20 PTC tissues, 20 HT with PTC tissues and 10 nodular goiter tissues).Results:① Statistical analysis showed that the expression of Wnt10a was significantly higher in PTC and HT with PCR tissues than in nodular goiter tissues (P<0.05) and the expression was significantly higher in PTC tissues than in HT with PTC tissues (P<0.05). ② The Wnt10a protein expression had significant difference between thyroid cancer and nodular goiter (P<0.05), but the expression did not have significant difference between PTC and HT with PTC (P>0.05). ③The expression of Wnt10a mRNA and protein had no significant relation with age, gender and lymph node metastasis in thyroid cancers (P>0.05) and only have relationship with pathological type.Conclusion:We identified that Wnt10a was differentially and highly expressed in PTC tissues and HT with PTC tissues. The study provided an effective early detection biomarker of PTC and hoping to contribute to the early diagnosis and pathological type determination of thyroid cancer.

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