| 柴路维 伊宏亮 蔡敏 包华琼.精子DNA损伤检测技术的研究进展[J].现代生物医学进展英文版,2015,15(18):3597-3600. |
| 精子DNA损伤检测技术的研究进展 |
| Progress on the Detection Techniques for DNA damage of Sperm |
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| DOI： |
| 中文关键词: 精子 DNA 损伤 检测技术 |
| 英文关键词: Sperm DNA damage Detection technique |
| 基金项目:重庆市科委重大科技攻关项目(cstc2013ggB00001)；重庆市科委基本业务经费（2013cstc-jbky-01707） |
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| 中文摘要: |
| 精子DNA完整性与男性生育力之间的关系是近些年来生殖医学研究领域的热点之一，精子DNA 损伤已成为反映男性生
育力的一个新指标。精子DNA的损伤原因有很多，有时可能是多种因素共同作用的结果。生殖系统疾病、环境污染、吸烟、微量元
素及各种理化因素等原因都可能导致精子DNA 完整性受损。常见的精子DNA完整性检测技术有原位末端标记法、精子染色体
扩散实验、精子染色质结构分析试验、单细胞凝胶电泳、荧光原位杂交技术和8-羟基脱氧鸟苷测定法等。随着检验技术的不断发
展，关于精子DNA 损伤的检测技术也在不断更新改进。本文主要就近十年来精子DNA 损伤机制、检测技术的相关研究进展作一
综述,提示现有的精子DNA 完整性检测技术尚不能满足临床和科研需要，急需找到一种理想的检测方法为男性不育的诊断和治
疗提供重要依据。 |
| 英文摘要: |
| The relationship between sperm DNA integrity and male fertility is one of hotspots in the field of reproductive
medicine in recent years. The sperm DNA damage has become a new index of the male fertility now. There are various reasons lead to
sperm DNA damage, sometimes the occurrence of it may be result of joint action of many factors. Reproductive system diseases,
environmental pollution, smoking, trace elements and a variety of physical and chemical factors and other reasons can cause sperm DNA
damage. The common methods for testing sperm DNA integrity are in situ end labeling method, sperm chromatin dispersion test, sperm
chromatin structure analysis test, Comet assay and fluorescence in situ hybridization technology and 8 - hydroxy - 2 - deoxyguanosine
measurement, etc. With the continuous development of test technology, detection technology of sperm DNA damage is also becoming
better and better. This article briefly reviews the research progress in decade on the mechanism of sperm DNA damage and detection
techniques, and the results suggest that the existing detection technology of sperm DNA integrity cannot satisfy the requirements of
clinical and scientific research. It is urgent to find an ideal detection method to provide evidences for the diagnosis and treatment of male
infertility. |
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