欢迎访问《现代生物医学进展》编辑部！

目的：比较乳腺癌细胞经过表柔比星处理前后的生物学行为，探讨表柔比星化疗对乳腺癌转移潜能的影响及机制。方法：人乳腺癌细胞MCF-7 和MDA-MB-231 分别给予正常培养和表柔比星6 小时处理，通过划痕实验和transwell 实验比较两组细胞迁移和侵袭能力的差别。MCF-7 细胞经过表柔比星处理不同时间后，通过real-time PCR 分析细胞中转移相关蛋白1(Metastasis Associated Protein 1, MTA1)表达水平的变化。建立小鼠4T1 乳腺癌模型，观察表柔比星化疗对小鼠肺表面乳腺癌转移灶的数量的影响。结果：划痕实验中，处理组MCF-7 和MDA-MB-231 细胞24 小时内平均划痕愈合距离均显著长于对照组细胞(P < 0.05)； transwell 实验中，处理组MDA-MB-231 细胞24 小时内穿膜细胞数显著多于对照组细胞(P<0.01)，MCF-7 细胞本身侵袭性低难以穿膜；real-time PCR结果显示，表柔比星处理使MCF-7 细胞中MTA1 转录水平出现显著上调(P < 0.05)；动物实验结果显示，处理组小鼠肺表面转移灶数量显著多于对照组(P < 0.01)。结论：表柔比星处理可以在体内和体外增强乳腺癌细胞的转移潜能，这一改变可能与其诱导MTA1的表达有关。

英文摘要:

Objective:To investigate the effect of epirubicin chemotherapy on metastatic potential of breast cancer and its possible mechanisms by comparison of the biological behaviors of epirubicin pre-treatment breast cancer cells with untreated cells.Methods:Human breast cancer MCF-7 and MDA-MB-231 cells were respectively treated with epirubicin for 6 hours or kept in normal culture medium. The migration and invasion capacity of these differently treated cells were assessed by wound-healing assays and transwell assays. Real-time PCR was used to analyze the alteration of mRNA levels of metastasis associated protein 1 (MTA1) in MCF-7 cells treated with epirubicin for different periods of time. Mouse 4T1 breast cancer model was established to observe the effect of epirubicin chemotherapy on the occurrence of surface lung metastases.Results:In the wound-healing assays, cells treated with epirubicin showed greater wound closure between 0 and 24 hours than untreated cells in both cell lines (P < 0.05). In the transwell assays, epirubicin treated MDA-MB-231 cells showed significant increase (P < 0.01) in the numbers of cells invading the Matrigel-coated membrane compared with untreated cells, but the difference in MCF-7 cells was not significant, probably due to their innate low invasive capacity. Real-time PCR showed that epirubicin treatment led to a significant increase (P < 0.05) in the expression of MTA1 mRNA in MCF-7 cells. In tumor-bearing mice, more surface lung metastases were observed in treated mice than untreated ones (P < 0.01).Conclusion:Epirubicin treatment can promote metastasis of breast cancer cells both in vitro and in vivo, and its stimulation of MTA1 is a possible mechanism.

View Full Text View/Add Comment Download reader