| 周春晶 刘玉凤 李宏宇 李军 孙秀英.菊花总黄酮对RSV感染A549细胞趋化因子的影响[J].现代生物医学进展英文版,2015,15(18):3435-3437. |
| 菊花总黄酮对RSV感染A549细胞趋化因子的影响 |
| Effects of ChrysanthemumDecoction on Chemokine of RSV Infection inA549 Cells |
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| DOI： |
| 中文关键词: 菊花总黄酮 RSV 感染 A549 细胞趋化因子 RANTES MCP-1 |
| 英文关键词: ChrysanthemumDecoction RSV infection A549 cell chemotactic factor RANTES MCP-1 |
| 基金项目:国家自然科学基金面上项目（30192101） |
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| 中文摘要: |
| 目的：探讨菊花总黄酮对小儿RSV 感染A549 细胞诱导RANTES 及MCP-1 释放作用影响。方法：实验分为细胞对照组，病
毒对照组，菊花总黄酮组和病毒唑组。在Hep-2 细胞和A549 细胞分别加入菊花总黄酮和病毒唑的含药维持液，测定上述两种药
物的最大无毒浓度；RSV 病毒感染Hep-2 细胞，观察药物对RSV的病毒抑制作用；RSV 感染A549细胞，ELISA 法测细胞趋化因
子RANTES及MCP-1含量。结果：菊花总黄酮50 %有效率优于病毒唑组，差异有统计学意义（P＜0.05）；RANTES 及MCP-1 释放
抑制作用比较中，菊花总黄酮组RANTES、MCP-1 明显降低，优于病毒唑组，差异有统计学意义（P＜0.05）。结论：菊花总黄酮能够
抑制RSV 病毒活性，明显降低A549 细胞释放RANTES、MCP-1，缓解患儿的呼吸道症状，对临床具有指导意义，值得临床推广。 |
| 英文摘要: |
| Objective:To investigate the effect of chrysanthemum Decoction on RANTES and MCP-1 release induced by RSV
infection in A549 cells.Methods:The experiment was divided into control group, virus control group, chrysanthemum Decoction group
and ribavirin group. Chrysanthemum Decoction and Ribavirin were added in Hep-2 cells and A549 cells containing maintenance
medium. The maximum non-toxic concentration of the two drugs was determined; after RSV infected Hep-2 cells, chrysanthemum
Decoction and virus of Polish containing maintenance solution was respectively added, and to observe the inhibitory effects of this drug
for A549 cells infected with RSV. Using ELISA method, the level of cell chemokine RANTES and MCP-1 was detected.Results:Total
flavonoids in chrysanthemum had an efficiency of 50 %, which was superior to ribavirin group, and the difference was statistically
significant (P<0.05); RANTES and MCP-1 decreased significantly in Chrysanthemum group, and the difference was statistically
significant compared with that of the ribavirin group (P<0.05).Conclusion:Chrysanthemumdecoction could inhibit RSV virus activity,
reduce the release of RANTES and MCP-1 in A549 cells. |
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