| Objective:To establish adult rat models with nutritional obesity by mixed feeding method.Methods:55 adult male
Sprague-Dawley (SD) rats were randomly divided into two groups: the control group (fed with normal diet, n=10) and the experimental
group (fed with mixed feeding, n=45). Two weeks after feeding, obesity-resistant rats were excluded from the experimental group. Body
weight (BW) was measured weekly. Nine weeks after feeding, body weight, body length, Lee's index, fasting blood glucose (FBG), serum
lipids, glucose tolerance and histomorphological changes of liver were observed.Results:The BWs of the experimental group and control
group were（645.6± 34.8）g and（483.3± 31.5）g, respectively, and the Lee's indexes of the two groups were 326.3± 24.2 and 302.7±
19.8, respectively. The BWs and Lee's indexes of experimental group were significantly higher compared with that of control group（P<0.
05）. Serum TG, TC, LDL-C, FBG, blood glucose after glucose tolerance of the experimental group were significantly higher than those
of control group. Pathological examination showed fatty degeneration in liver of the experimental group but there was no difference in
the control group.Conclusion:Adlut rats models with nutritional obesity, which is associated with decreased glucose tolerance and
hyperlipidemia, can be built using mixed feeding successfully and stably. |
