| 翟艺宗 黄昌林 常祺 王久清 张佳 郭延岭.脉冲电流经皮刺激肝区对运动性疲劳大鼠肝脏线粒体呼吸链酶复合物活性的影响[J].现代生物医学进展英文版,2015,15(17):3236-3239. |
| 脉冲电流经皮刺激肝区对运动性疲劳大鼠肝脏线粒体呼吸链酶复合物活性的影响 |
| Influence of Percutaneous Impulsive Current Stimulation of Hepatic Regionon the Hepatic Mitochondria, Respiratory Chain Enzyme Complex Activity inExercise-induced Fatigue Rats |
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| DOI： |
| 中文关键词: 脉冲电流刺激 运动性疲劳 肝脏线粒体 呼吸链酶复合物活性 |
| 英文关键词: Impulsive current stimulation Exercise-induced fatigue Hepatic mitochondria Respiratory chain enzyme complex
activity |
| 基金项目:全军卫生和疾病防控应用性研究课题计划(13BJYZ18)；
新乡医学院2013 年硕士研究生科研创新支持计划项目（YJSCX201344Y） |
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| 中文摘要: |
| 目的：探讨脉冲电流经皮刺激肝区对运动性疲劳大鼠肝脏线粒体呼吸链酶复合物（I~IV）活性的影响。方法：选取健康8 周
龄Wistar雄性大鼠80 只为实验对象，将实验动物随机分为安静对照组(A 组)、疲劳对照组（B组）、疲劳前刺激组(C 组)、疲劳后刺
激组(D 组)，造模成功后分别在1、3、5 周末分批处死。采用分光光度法测定1、3、5 周各组动物的肝脏线粒体呼吸链酶复合物
（I~IV）活性，大鼠肝脏线粒体蛋白定量用Bradfood蛋白定量法测定。结果：第3、5 周末D 组和B 组、C 组大鼠游泳力竭时间差异
比较具有统计学意义(P＜0.05)。A 组、C 组及D 组大鼠肝脏线粒体呼吸链酶复合物（I~IV）活性水平在第3、5 周末均高于B 组
(P＜0.05)。D 组大鼠肝脏线粒体呼吸链酶复合物（I~IV）活性水平在第3、5 周末均高于C 组(P＜0.05)。结论：脉冲电流经皮刺激运
动性疲劳大鼠肝区可以提高其氧化呼吸链酶复合物（I~IV）的活性水平。 |
| 英文摘要: |
| Objective:To discuss the effects of percutaneous impulsive current stimulation of hepatic region on the hepatic
mitochondria, respiratory chain enzyme complex （I~IV）activity in exercise-induced fatigue rats and further investigate the
exercise-induced fatigue.Methods:Eight-week-old Wistar male rats were selected to be experimental animals which were randomly
separated into four groups: control group (group A); fatigue group (group B); stimulation group before fatigue (group C) and stimulation
group after fatigue (group D). At the weekend of 1st, 3rd and 5th week after modeling, rats were chosen fromeach group and sacrificed. We
adopted spectrophotometry to determine hepatic mitochondria respiratory chain enzyme complex（I~IV）activity of the rats in each group
in 1st, 3rd and 5th week. As for the protein quantification of the hepatic mitochondria in rats, Bradfood protein quantification was adopted.Results:At the weekend of 3rdand 5thweek, the exhausting time of group B was less than that of group C and group D, and the difference
among these groups in swimming exhausting time was statistically significant (P<0.05). The level of activity of hepatic mitochondria
respiratory chain enzyme complex（I~IV）in group A, group C and group D in the weekend of 3rd week and 5th week was higher than that
in group B and the difference was statistically significant(P<0.05). The level of activity of hepatic mitochondria respiratory chain enzyme
complex（I~IV）in group D in the weekend of 3rdweek and 5th week was higher than that in group C and the difference was statistically
significant (P<0.05).Conclusion:Percutaneous impulsive current stimulation of hepatic region in exercise-induced fatigue rats may
improve the activity of the oxidant respiratory chain enzyme complex（I~IV）. |
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