| 程昱 王松柏 姚红 樊健 卢宝玲 钟丽华 于雷 朱丽影△.硒对甲型H1N1 流感病毒感染小鼠的保护作用[J].现代生物医学进展英文版,2015,15(17):3220-3222. |
| 硒对甲型H1N1 流感病毒感染小鼠的保护作用 |
| Protection from H1N1 Influenza Virus Infections in Mice bySupplementation with Selenium |
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| DOI： |
| 中文关键词: 硒 流感病毒 TNF-alpha IFN-r |
| 英文关键词: Selenium Influenza virus TNF-alpha IFN-r |
| 基金项目:黑龙江省博士后资助项目（LBH-Z12195）；黑龙江省教育厅科学技术项目（12521294） |
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| 中文摘要: |
| 目的：通过研究硒对流感病毒悬液滴鼻处理的小鼠的体重变化、死亡率、血清硒水平和细胞因子的影响，探讨硒对感染流感
病毒小鼠的保护作用。方法：将60 只昆明小鼠分为5 组，每组12 只，分别为缺硒组(0 mg/kg)、正常给硒组(0.2 mg/kg)、补充硒组
(0.3 mg/kg)、补充硒组(0.4 mg/kg)、补充硒组(0.5 mg/kg)。给5 周龄的小鼠滴鼻接种50 uL的A/NWS/33(H1N1)病毒悬液并观察21
天，监测每组小鼠的体重变化和死亡率；并在接种病毒后的第3 天、第5天，检测小鼠的血清硒、TNF-alpha和IFN-r水平。结果：缺硒
组小鼠的死亡率高于正常给硒组和补充硒组(P<0.05)；缺硒组小鼠的血清硒水平明显低于正常给硒组和补充硒组(P<0.05)；在病
毒感染第5 天，缺硒组小鼠的TNF-alpha和IFN-r含量低于正常给硒组和补充硒组(P<0.05)，差异均有统计学意义。结论：硒可以提高
机体对抗流感病毒的免疫反应。 |
| 英文摘要: |
| Objective:To explore protective effects of supplemental selenium in mice inoculated intranasally with suspensions of
influenza virus by investigating the effects of supplemental selenium on weight loss and recovery, mortality, serum selenium levels and
cytokine of mice.Methods:60 Kunming mice were divided into five groups with 12 mice in each one, Se-deficient group (0 mg/kg),
Se-adequate group (0.2 mg/kg), Se-supplemented group (0.3 mg/kg), Se-supplemented group (0.4 mg/kg), Se-supplemented group (0.5
mg/kg). Mice (5 weeks old) were inoculated intranasally with 50 uL of viral suspension and observed for 21 days to monitor body weight
change and mortality. The serum content of TNF-alpha, IFN-r and selenium were detected at 3 and 5 days after virus inoculation.Results:The mortality in mice of Se-deficient group was higher than that of Se-adequate group and Se-supplemented group (P<0.05). The lever of
serumseleniumin mice of Se-deficient group was much lower than that of Se-adequate group and Se-supplemented group (P<0.05). The
concentration of TNF-alpha and IFN-r in mice of Se-deficient group was lower than that of Se-adequate group and Se-supplemented group at
5 days after virus inoculation (P<0.05), the differences were all statistically significant.Conclusion:The data indicates that selenium
supplementation may provide a feasible approach to improving the immune response to lethal influenza infection. |
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