| 毕长龙 胡玉华 栾嵩 王吉锡 李波.人脐带间充质干细胞移植对糖尿病大鼠血清学的影响[J].现代生物医学进展英文版,2015,15(16):3019-3021. |
| 人脐带间充质干细胞移植对糖尿病大鼠血清学的影响 |
| Study on the Effect of Human Umbilical Cord Mesenchymal StemCellTransplantation on Serology of Diabetes in Rats |
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| DOI： |
| 中文关键词: 人脐带间充质干细胞 糖尿病 大鼠 血清学 |
| 英文关键词: Human umbilical cord mesenchymal stemcells Diabetes Rat Serology |
| 基金项目:黑龙江省教育厅科学技术研究项目（12531319） |
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| 中文摘要: |
| 目的：观察人脐带间充质干细胞(huMSCs)移植对糖尿病大鼠血糖、胰岛素和血清因子表达的影响。方法：随机选择12 只
Wistar 大鼠通过腹腔注射链脲佐菌素50 mg/kg，血糖高于16.7 mmol/L 者定为糖尿病大鼠，再将其随机分为糖尿病组和干细胞
组，每组6 只，同时选择6 只雄性Wistar 大鼠为正常组。干细胞组大鼠腹腔注射huMSCs 细胞悬液，糖尿病组注射PBS液。分别于
注射2 周、4 周和6周后测定和比较各组大鼠的血糖、血清胰岛素、肿瘤坏死因子(TNF- alpha)和白介素-6(IL-6)的表达。结果：与正常
组比较，糖尿病组和干细胞组大鼠的血糖水平均显著升高，胰岛素水平均显著降低(P<0.01)。与糖尿病组比较，干细胞组大鼠注射
huMSCs后的血糖水平明显降低，胰岛素水平明显升高(P<0.05)，血清TNF-alpha和IL-6 mRNA水平均显著降低(P<0.01)。结论：
huMSCs移植能显著降低糖尿病大鼠的血糖，促进其胰岛素分泌，同时降低血清TNF-琢和IL-6 的表达。 |
| 英文摘要: |
| Objective:To observe the effect of human umbilical cord mesenchymal stemcell (humscs) transplantation on serology
of diabetes in rats.Methods:12 male Wistar rats were randomly selected and were injected with streptozotocin (50 mg/kg)
intraperitoneally. The blood glucose of successful diabetic rat was higher than 16.7 mmol/L. 12 rats were randomly divided into diabetic
group (n=6) and stemcell group (n=6). At the same time, 6 male Wistar rats were selected as normal group. The rats of stem cell group
and diabetic group were injected with huMSCs cell suspension and PBS solution respectively. The levels of blood glucose, seruminsulin,
tumor necrosis factor alpha (TNF-alpha) and interleukin -6 (IL-6) were measured and compared at 2 weeks, 4 weeks and 6 weeks after
transplantation.Results:The blood glucose levels of rats were significantly higher in diabetic group and stem cell group than that in
normal group, while the insulin level of both groups were significantly lower (P<0.01); compared with that in diabetic group, the blood
glucose level was obviously reduced in rats of stem cell group after injection with huMSCs, while the insulin level was significantly
elevated and the expression of TNF-alpha and IL-6 mRNA were markedly decreased (P<0.05).Conclusion:The transplantation of huMSCs
could evidently reduce the blood glucose of diabetic rats, increase the insulin secretion, and decrease the serumTNF-alpha and IL-6 levels. |
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