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目的：观察楤木皂苷（total saponins extracted fromAralia taibaiensis，sAT）对大鼠心肌缺血/再灌注（myocardia1 ischemia/ reperfusion，MI/R）损伤的影响。方法：可逆性冠脉左前降支结扎缺血30 min 再灌注3 h 复制MI/R 模型，将SD 大鼠随机分为假手术组、模型组、sAT 低、中、高剂量组，每组10 只。采用伊文思蓝（EB）、2,3,5- 氯化三苯基四氮唑蓝（TTC）双染法测定心肌梗死面积，苏木精- 伊红（HE）染色法观察心肌病理学形态变化，并检测血清中乳酸脱氢酶（LDH）、肌酸激酶同工酶（CK-MB）、超氧化物歧化酶（SOD）、丙二醛（MDA）、过氧化氢酶（CAT）及谷胱甘肽过氧化物酶（GSH-Px）水平。结果：与模型组比较，sAT 中、高剂量组可明显缩小心肌梗死面积（P<0.05），并显著降低血清中LDH、CK-MB 及MDA 的含量，同时使得血清中SOD、CAT 和GSH-Px 的活性增加。且所有给药组心肌组织的病理损伤也小于模型组。结论：sAT 对大鼠MI/R 损伤具有保护作用，其机制可能与抗氧化作用相关。

英文摘要:

Objective:To investigate the protective effects of total saponins extracted from Aralia taibaiensis (sAT) on myocardial ischemia/reperfusion injury in rats.Methods:An in vivo model of myocardial ischemia 30 min followed by 3 h reperfusion was made by reversibly ligating coronary left descending artery. The male SD rats were divided randomly into shamgroup; ischemia/reperfusion model group; sAT (60 mg·kg-1; 120 mg·kg-1; 240 mg·kg-1) groups (n=10). The area of myocardial infarction and the change of histological tissue were assessed after 3 h reperfusion. Meanwhile, the levels of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB), superoxide dismutase (SOD), Malondialdehyde (MDA), catalase (CAT) and glutathione peroxidase (GSH-Px) in serumwere determined by assay kits.Results:Compared with model group, pretreatment with sAT significantly reduced infarct size, decreased the levels of LDH, CK-MB and MDA in the serum, and increased the activities of SOD, CAT and GSH-Px. In addition, sAT improved the pathologic changes of myocardium.Conclusion:sAT has cardioprotective effect against MI/R injury. The mechanism may be related to its antioxidant activities.

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