| 张哲 郭昊 董加强 蒋孝明 聂勇战 樊代明.长链非编码RNA UCA1 在胃癌多药耐药中的作用研究[J].现代生物医学进展英文版,2015,15(15):2811-2814. |
| 长链非编码RNA UCA1 在胃癌多药耐药中的作用研究 |
| The Role of lncRNA UCA1 on Drug Resistance in Gastric Cancer |
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| DOI： |
| 中文关键词: 长链非编码RNA UCA1 胃癌 多药耐药 |
| 英文关键词: LncRNA UCA1 Gastric cancer Multi-drug resistance (MDR) |
| 基金项目:国家自然科学基金重点项目（81030044） |
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| 中文摘要: |
| 目的：研究胃癌耐药细胞及其亲本细胞中长链非编码RNA UCA1 的表达差异，探讨UCA1 在胃癌多药耐药中的作用。方
法：通过实时荧光定量PCR(qRT-PCR)检测胃癌耐药细胞SGC7901/ADR、SGC7901/VCR 及其亲本细胞SGC7901 中UCA1 的表
达差异；通过siRNA转染降低SGC7901/ADR 中UCA1表达，MTT法检测细胞半数抑制浓度(IC50)的变化，流式细胞仪检测细胞
凋亡变化。结果：QRT-PCR 结果显示，UCA1 在SGC7901/ADR 和SGC7901/VCR胃癌耐药细胞表达显著高于SGC7901胃癌亲本
细胞；MTT 实验表明，干扰UCA1 的SGC7901/ADR相对于阴性对照(NC)组的IC50 显著降低；凋亡检测结果显示，在相同剂量化
疗药物作用下，干扰UCA1 后SGC7901/ADR 凋亡率显著高于NC组；Western blot证实，干扰UCA1 表达可显著降低BCL-2 蛋白
表达。结论：长链非编码RNA UCA1 在胃癌耐药细胞表达显著升高，干扰UCA1表达可明显逆转胃癌耐药，UCA1 可作为治疗胃
癌耐药的重要分子靶标。 |
| 英文摘要: |
| Objective:To investigate the differential expression of lncRNA UCA1 in SGC7901/ADR, SGC7901/VCR and
SGC7901 gastric cancer cell lines and to explore its role in multi-drug resistance (MDR) of gastric cancer (GC).Methods:The expression
level of UCA1 in SGC7901/ADR, SGC7901/VCR and SGC7901 cell lines was obtained by quantitative real-time PCR (qRT-PCR) detection.
SiRNA transfection was applied to knockdown UCA1 expression in SGC7901/ADR. The drug sensitivity (IC50) of SGC7901/ADR
were detected byMTT assay; apoptosis rates were detected by flowcytometry and the protein expression of Bcl-2 by western blot. Results:QRT-PCR results showed that the expression level of UCA1 in SGC7901/ADR and SGC7901/VCR was significantly higher than that in
parental SGC7901 cells. The MTT assay and flow cytometry analysis showed that the drug sensitivity (IC50) and apoptosis rates of
SGC7901/ADR were significantly increased compared with negative control (NC) group. Western blot results confirmed that knockdown
of UCA1 in SGC7901/ADR can significantly reduce the expression of BCL-2 protein.Conclusion:Long non-coding RNA UCA1 was
significantly up-regulated in two MDR GC cell sublines, SGC7901/ADR and SGC7901/VCR. Our findings indicate that UCA1 plays a
positive role in the regulation of the MDR phenotype of GC MDR cell sublines and is a potential target to resverse GC MDR. |
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