| 王志华 邹丽丽 张巧梅 吴畏 李健楠 张丽娜 孟尽海.Orexin-A介导histamine能神经系统促进氯胺酮麻醉觉醒[J].现代生物医学进展英文版,2015,15(14):2663-2665. |
| Orexin-A介导histamine能神经系统促进氯胺酮麻醉觉醒 |
| Orexin-A Facilitates Emergence fromKatemine Anesthesia throughHistaminergic System |
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| DOI： |
| 中文关键词: orexin-A orexin-B 诱导时间 觉醒时间 |
| 英文关键词: Orexin-A Orexin-B LORR RORR |
| 基金项目:国家自然科学基金青年基金项目（81401138）；宁夏医科大学科学研究基金面上项目（XM201321） |
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| 中文摘要: |
| 目的：探索氯胺酮麻醉下，Orexin 神经信号是否激活结节乳头体核（Tuberomammillary Nucleus，TMN）促进大鼠氯胺酮麻
醉觉醒。方法：成年雄性SD 大鼠（体重230-280 g），在10%水合氯醛麻醉下（1 ml/kg，i.p.）进行以下实验：①TMN 核团埋置微注射
外套管，回笼单独饲养7 天后，大鼠随机分为三组，分别为对照组（NS）、orexin-A 组与orexin-B组。TMN 核团分别双侧微注射NS
（0.3 滋L）、orexin-A（100 pmol/0.3 滋L）及orexin-B（100 pmol/0.3 滋L）观察氯胺酮麻醉下(100 mg/kg, 腹腔注射)大鼠诱导时间与觉醒
时间；②上述实验7 天后，大鼠随机分为三组，分别为溶剂DMSO 组、SB334867 组与TCS-OX2-29 组，TMN 核团分别双侧微注射
DMSO（0.3 滋L）、orexin 1 型受体（the orexin type 1 receptor，OX1R）的拮抗剂SB334867（20 滋g/0.3 滋L）和orexin 2型受体（the
orexin type 2 receptor，OX2R）的拮抗剂TCS-OX2-29（20 滋g/0.3 滋L）观察氯胺酮麻醉下大鼠诱导时间与觉醒时间。结果：①各组大
鼠的诱导时间无统计学差异。②在TMN 核团微注射orexin-A 与对照组相比明显缩短了大鼠的觉醒时间（43.17± 6.31 min vs
51.17± 4.45 min，P<0.05），而微注射orexin-B 与对照组相比并没有明显影响大鼠的觉醒时间（50.33± 3.50 min vs 51.17± 4.45
min，P>0.05）。③TMN 核团微注射OX1R 拮抗剂SB334867 较溶剂DMSO 组延长了麻醉觉醒时间（60.83± 8.84 min vs 49.00±
5.73 min，P<0.05），OX2R拮抗剂TCS-OX2-29 与溶剂DMSO组相比并没有明显影响大鼠的觉醒时间（50.83± 4.79 min vs 49.00±
5.73 min，P>0.05）。结论：本研究实验证据证实在氯胺酮麻醉下，orexin 神经信号可能通过激活TMN 区组胺能神经系统促进麻醉
向觉醒的转换。 |
| 英文摘要: |
| Objective:The aim of this study was to test whether activation of orexin signal in Tuberomammillary Nucleus (TMN)
can facilitate emergence from katemine anesthesia or not.Methods:Adult male SD rats (body weight 230-280 g) were anesthetized
by10% chloral hydrate (1 ml/kg, ip) to do the following work. ① Guide cannulas for microinjection to the TMN were embedded. Then
the rats were fed in separate cage. Seven days later, rats were randomly divided into three groups. And then orexin-A (100 pmol/0.3 uL) ,
orexin-B (100 pmol/0.3 uL) and and their solvent NS (0.3 uL) were microinjected into TMN respectively to observe the induction time
and awakening time in rats. ② The same operation as step 1, Seven days later, rats were grouped randomly. And then the OX1R
antagonist SB334867 (20 uL/0.3 uL), OX2R antagonist TCS-OX2-29 and their solvent DMSO (0.3 uL) were microinjected into TMN
respectively to observe the time of induction and awakening in rats.Results:① No statistical difference of induction time was found in
each group. ②The rats which injected orexin-A (100 pmol/0.3 uL) recovered much faster from ketamine anesthesia (100 mg/kg, ip) than
controlled group (injected NS 0.3 uL) （43.17± 6.31 min vs 51.17± 4.45 min，P<0.05）.There was no significant difference on the
emergence from ketamine anesthesia between the rats received orexin-B and controlled rats (50.33± 3.50 min vs 51.17± 4.45 min,
P>0.05). ③ The injection of OX1R antagonist SB334867 (20 uL/0.3 uL) to TMN could prolong the emergence time from ketamine
anesthesia compared to the solvent DMSO (0.3 uL) group (60.83± 8.84 min vs 49.00± 5.73 min, P<0.05). The injection of OX2R
antagonist TCS-OX2-29 did not significantly affect the rat emergence time (50.83± 4.79 min vs 49.00± 5.73 min, P>0.05).Conclusion:Orexin-A Can facilitates emergence awakening fromketamine anesthesia through histaminergic systemin TMN. |
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