| 杨渝佳 蔡越 张小群 胡佳勇 周林 曾春雨.血浆HOTAIR 在冠状动脉粥样硬化性心脏病的表达及诊断价值[J].现代生物医学进展英文版,2015,15(14):2617-2620. |
| 血浆HOTAIR 在冠状动脉粥样硬化性心脏病的表达及诊断价值 |
| Expression of plasma HOTAIR in Coronary Artery Heart Disease andIts Diagnostic Value |
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| DOI： |
| 中文关键词: 长链非编码RNA HOX 转录反义RNA 生物标志物 冠状动脉粥样硬化性心脏病 |
| 英文关键词: Long non-coding RNA HOX transcript antisense RNA Biomarker Coronary heart disease |
| 基金项目:国家自然科学基金重点项目(31130029) |
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| 中文摘要: |
| 目的：检测血浆HOX 转录反义RNA(HOTAIR)表达水平，并初步探讨血浆HOTAIR 作为冠状动脉粥样硬化性心脏病(CAD)
标志物的诊断价值。方法：CAD患者和非CAD 患者分别作为本研究的疾病组和对照组，血标本来自重庆大坪医院心内科介入室
（2013 年1 月-9 月）。运用实时荧光定量聚合酶链反应（qRT-PCR）分别检测疾病组和对照组各100 例血浆HOTAIR 水平，同时对
上述血浆HOTAIR 的表达值绘制受试者工作特征（ROC）曲线，并计算ROC 曲线下面积（AUC）。结果：与对照组(0.25± 0.10)相
比，HOTAIR 在CAD患者血浆(0.80± 0.30)中表达显著升高(P＜0.05)；ROC曲线分析表明，AUC为0.776，Cut-off 值为0.55 时，诊
断CAD的灵敏度为75%，特异度为80%。结论：冠状动脉粥样硬化性心脏病患者血浆HOTAIR 水平升高，血浆HOTAIR可作为
诊断冠状动脉粥样硬化性心脏病的独立生物标志物。 |
| 英文摘要: |
| Objective:To explore the expression of HOX transcript antisense RNA (HOTAIR) in coronary artery heart disease
(CAD) and its clinical value as biomarker of CAD.Methods:100 CAD patients and 100 non CAD patients were from the interventional
treatment roomof Cardiovascular Department in Daping Hospital (01/2013-09/2013). HOTAIR expression was quantified by quantitative
real-time PCR, respectively. The ability to distinguish the CAD group from control group was characterized by receiver operating
characteristic (ROC) curve, the area under ROC curve (AUC) was calculated.Results:Compared with the control group (0.25± 0.10),
the expression of HOTAIR was increased significantly in CAD patients (0.80± 0.30) (P<0.05), ROC curve analysis showed that the AUC
was 0.776, the Cut-off value was 0.55, the corresponding sensitivity and specificity in the diagnosis of CAD was 75% and 80% ,
respectively.Conclusion:Plasma level of HOTAIR was significantly increased in patients with CAD, suggesting that it can be served as
an independent biomarker for CAD. |
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