| 周敏 李宁 付艳 杜崇涛 陈巍 杨勇军.poly(dA:dT)促进体外培养的人绒毛组织AIM2 炎性体活化[J].现代生物医学进展英文版,2015,15(14):2614-2616. |
| poly(dA:dT)促进体外培养的人绒毛组织AIM2 炎性体活化 |
| poly(dA:dT) Actives AIM2 Inflammasome in Human PlacentalVilli in Vitro |
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| DOI： |
| 中文关键词: 人胎盘绒毛组织 AIM2 炎性体 Caspase-1 IL-1beta |
| 英文关键词: Human Placental Villi AIM2 Inflammasome Caspase-1 IL-1beta |
| 基金项目:国家自然科学基金项目(31172298；31372410)；长江学者和创新团队发展计划(IRT1248) |
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| 中文摘要: |
| 目的：在妊娠过程中，胎盘可能暴露于多种病原微生物，威胁胎儿正常生长发育。为探讨人胎盘绒毛组织是否表达AIM2 炎
性体成员基因以及人胎盘组织的AIM2 炎性体的活化形式。方法：以THP-1 细胞来源的RNA 和蛋白作为阳性对照，分别应用
RT-PCR和Western blot方法检测人早孕期胎盘绒毛组织中AIM2 炎性体两个相关基因AIM2 和ASC 的表达。分离和体外培养
人胎盘绒毛膜组织，并用不同浓度的poly(dA:dT)进行转染，处理24小时后，分别收集组织培养上清和蛋白裂解液，Western blot
检测蛋白裂解液中caspase-1 的活化，ELISA 检测培养上清中IL-1beta的分泌。结果：RT-PCR和Western blot 结果均显示人早孕期
胎盘绒毛组织组成性表达AIM2 炎性体相关基因AIM2 和ASC。同时，体外培养的人胎盘绒毛组织在转染5 ug/mL poly(dA:dT)
后，caspase-1 剪切片段p10 显著增多，培养上清中IL-1beta分泌也显著增多（P<0.01）。结论：人胎盘绒毛组织存在功能性的AIM2炎
性体，能够被胞内双链DNA 活化。 |
| 英文摘要: |
| Objective:During pregnancy, the placenta may become exposed to microorganisms, which may pose a potential threat
to the embryo/fetus well-being. This study was preformed to investigate expression and function of the AIM2 inflammasome in human
placental villi.Methods:The expression of AIM2 and ASC, two components of the AIM2 inflammasome, were determined in human
placental villi by RT-PCR and Western blot. RNA and protein from THP-1 cells were used as positive control. Human placental villi
were isolated and cultured in vitro, then transfected with different doses of poly (dA:dT) for 24 hours. The supernatant were collected for
analysis of IL-1beta secretion and tissue lysates were harvested for detection of caspase-1 activation by Western blot.Results:RT-PCR and
Western blot analysis showed that ASC and AIM2 were constitutively expressed in human placental villi. Furthermore, treatment with 5
ug/mL poly (dA:dT) markedly induced IL-1beta release and increased caspase-1 activity.Conclusion:The AIM2 inflammasome is
expressed and may plays an important role in human placental villi, which is activated by cytosolic double-stranded DNA stimulation. |
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