| 王天真 赵然 韩昌松 张玉华 叶盛前.乙型肝炎病毒4 种基因型与干扰素治疗应答的关系[J].现代生物医学进展英文版,2015,15(13):2451-2453. |
| 乙型肝炎病毒4 种基因型与干扰素治疗应答的关系 |
| The Relationship between Four Genotypes of HBV and the Response toInterferon |
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| DOI： |
| 中文关键词: HBV 基因型 干扰素 |
| 英文关键词: HBV Genotype Interferon |
| 基金项目:黑龙江省卫生厅科研课题（2012-750） |
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| 中文摘要: |
| 目的：在细胞水平上比较乙型肝炎病毒（HBV）基因型A, B, C, D对干扰素治疗的不同应答反应，进而探讨基因型对干扰素
治疗效果的影响。方法：利用脂质体法将前期构建好的HBV 基因型A, B, C, D质粒分别转染入HepG2 细胞系中，并在转染细胞
上清中加入干扰素-alpha2a（IFN-alpha-2a）。ELISA 方法用来检测上清中HBV抗原，荧光定量PCR 方法检测上清中HBV DNA，通过比较
加药前后上清中HBV 抗原和DNA水平的变化，反应HBV基因型对IFN-琢2a 的治疗反应。结果：HBV 4种基因型对IFN-琢2a 治
疗的应答反应存在差异，其中A 和B 基因型对IFN-琢2a 的应答明显高于C 和D基因型；而基因型A 和B 之间、以及基因型C 和
D 之间对IFN-alpha-2a 的反应无统计学差异。结论：HBV基因型能影响干扰素的治疗效果，其中A 和B 基因型对IFN-alpha-2a 的治疗反
应优于C 和D 基因型，在临床上应开展HBV 基因分型检测，用以指导临床用药的选择。 |
| 英文摘要: |
| Objective:The aim of this study was to compare the response of four genotypes of HBV to interferon in cellular level
and analyze the influence of HBV genotype on therapeutic efficacy of interferon.Methods:The plasmids with A, B, C. and D genotype
HBV were transfected into HepG2 cells using liposome method. Add interferon into the supernatant, test the levels of HBV antigens by
ELISA and DNA by quantitative PCR, then compare the changes of HBV antigens and DNA between interferon treated and un-treated
groups, which can show the response of four types of HBV to interferon.Results:A, B, C. and D genotype HBV showed different response
to interferon. The effect of interferon on A and B genotype HBV was more apparent than C and D genotype HBV, but there was
no difference between A and B, also between C and D.Conclusion:HBV genotype can influence the response of interferon. Genotyping
was important for the choice of antiviral drug in the clinic treatment. |
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