| 周淑琴 陈群力 熊野娟 唐浩.柱前衍生HPLC 法测定大鼠血浆中的河蚌糖胺聚糖[J].现代生物医学进展英文版,2015,15(13):2447-2450. |
| 柱前衍生HPLC 法测定大鼠血浆中的河蚌糖胺聚糖 |
| Determination of Anodonta Glycosaminoglycans in Rat Plasma by PrecolumnDerivatization High Performance Liquid Chromatography |
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| DOI： |
| 中文关键词: 河蚌糖胺聚糖 柱前衍生高效液相色谱法 异硫氰酸荧光素 荧光检测器 |
| 英文关键词: Anodonta glycosaminoglycans Precolumn derivatization HPLC FITC FLD |
| 基金项目:上海高职高专重点专业"085工程"飞跃计划项目（FY(13)400-A5-1-01） |
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| 中文摘要: |
| 目的：建立柱前衍生高效液相色谱法(HPLC) 用于河蚌糖胺聚糖在大鼠血浆中的含量测定。方法：使用异硫氰酸荧光素
（FITC）对河蚌糖胺聚糖进行荧光标记，色谱柱为Shodex SB-804HQ(8.0× 300 mm)，流动相为流速为0.5 mL·min-1的0.1 mol·L-1
的NaH2PO4-Na2HPO4缓冲液（pH 7.8），柱温30℃，激发波长495 nm，发射波长520 nm。结果：FITC对河蚌糖胺聚糖的荧光标记率
为70；线性范围为5～120 滋g·mL-1，批内和批间精密度均小于15.0%，提取回收率为51.5～58.6%，样品在血浆中稳定性良好。结
论：血浆中内源性杂质不干扰样品的测定，建立的方法符合生物样本的分析要求。 |
| 英文摘要: |
| Objective:To establish a precolumn derivatization HPLC method for the determination of anodonta glycosaminoglycans
in rat plasma.Methods:FITC was used as the flurocescent labeling reagent of anodonta glycosaminoglycans. The separation was
carried on a Shodex SB-804 HQ column (8.0× 300 mm) and 0.1 mol·L-1 sodium dihydrogen phosphate-disodium hydrogen phosphate
buffer was employed as the mobile phase. The flow rate was 0.5 mL·min-1 and the column temperature was set on 30 ℃. Flurocescence
detection was used with the excitation wavelength at 495 nm and the emission wavelength at 520 nm.Results:The flurocescent labeling
ratio was 70. The linear range of flurocescent labeling anodonta glycosaminoglycans was 5-120 ug·mL-1. The RSD of intra- and interbatch
precision were ≤ 15.0%. The extraction recovery was 51.5-58.6%and the sample was stable in plasma.Conclusion:There was no
significant interference fromendogenous substances and the method can be applied to determine the anodonta glycosaminoglycans in rat
plasma. |
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