| Objective:To explore the effect of Gastrodin on cell viability and apoptosis of SGC-7901 in vitro.Methods:SGC-7901
cells were cultured and then were randomly divided into normal, control and experimental groups. According to different effecting time,
they were further divided them into 6, 12, 24, 48, 72 h, 5 subgroups. Each subgroup was given 8 well cells. The experimental group received
2 mg/mL Gastrodin, control group was given saline, normal group had no other treatment. The cell viability was detected in different
time points by MTT, TUNEL method was used for apoptosis detection.Results:Gastrodin inhibited the cell proliferation and promoted
cell apoptosis, and these effects had time dependency. Cell viability and apoptosis between normal and control group had no statistical
significance (P>0.05). At 6 h, 12 h, 24 h, 48 h, 72 h time points, the cell viability of experimental group was respectively 0.41± 0.05,
0.42± 0.07, 0.43± 0.06, 0.47± 0.07, 0.52± 0.08, while that was respectively 0.44± 0.07, 0.49± 0.12, 0.50± 0.09, 0.69± 0.11, 0.72±
0.12 in control group. The cell viability of experimental group was lower than that of control group（P<0.05）. The apoptotic index of experimental
group was respectively 7.12± 0.65, 10.48± 1.26, 15.37± 1.69, 17.52± 2.33, 33.62± 3.65, while that was respectively 1.08±
0.05, 3.41± 0.56, 6.09± 0.91, 9.52± 1.14, 11.57± 1.18 in control group. Compared with control group, the apoptotic index of experimental
group was profoundly higher (P<0.05).Conclusion:Gastrodin can decrease the gastric cancer SGC-7901 cell viability and increase
cell apoptosis, which may have anti-tumor effect. |
