| 卜雯雯1 朱欣婷1,2 刘云2,3 晏容1,2 郭侃1 张云辉4 李晓飞.斑蝥素酸镁与斑蝥酸钠对喉癌Hep-2 细胞抑制活性比较的初步研究[J].现代生物医学进展英文版,2015,15(12):2234-2237. |
| 斑蝥素酸镁与斑蝥酸钠对喉癌Hep-2 细胞抑制活性比较的初步研究 |
| A Tentative Comparative of the Inhibitory Effects of MagnesiumCantharidate and SodiumCantharidate on Hep-2 Cells |
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| DOI： |
| 中文关键词: 斑蝥素酸镁 斑蝥酸钠 人喉癌上皮细胞 SRB染色法 抗肿瘤 集落形成 |
| 英文关键词: Magnesium Cantharidate Sodium Cantharidate Human laryngeal epithelial cells (Hep-2) SulforhodamieB (SRB)
assay Anti-tumor Cell colony forming experiment |
| 基金项目:国家自然科学基金项目（81260488）；贵州省科技厅社会发展攻关项目（黔科合SY 字[2011]3031）;
贵州省科技厅社会发展攻关项
目（黔科合SY字[2012]3082）；贵州省教育厅特色重点实验室建设项目（黔教合KY 字[2014]212) |
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| 中文摘要: |
| 目的：探讨自主研发产物--斑蝥素酸镁对人喉癌上皮细胞的增殖抑制活性是否好于临床抗癌药物斑蝥酸钠。方法：采用磺
酰罗丹明染色法（SRB 法）、细胞集落形成实验分别考察斑蝥素酸镁和斑蝥酸钠对人喉癌上皮细胞Hep-2 的影响。结果：与斑蝥酸
钠相比，斑蝥素酸镁作用喉癌Hep-2 细胞的效果更加显著。其IC50仅为2.19 umol·L-1，远低于斑蝥酸钠的IC50（15.75 umol·L-1）。
细胞集落形成实验显示，与斑蝥酸钠相比，斑蝥素酸镁明显阻止细胞集落的形成，最低有效浓度为1.75 umol·L-1，远低于斑蝥酸钠
的最低有效浓度（3.50 umol·L-1）。结论：与斑蝥酸钠相比，斑蝥素酸镁对喉癌Hep-2 细胞具有更加显著的抑制效果，可进行更加深
入的研究。 |
| 英文摘要: |
| Objective:To discuss whether the self-developed magnesium cantharidate possesses better anti-proliferation activity in
Hep-2 cells linethan Sodium Cantharidate.Methods:The sulforhodamieB (SRB) assay and cell colony forming tests were employed to
detect the influence of Magnesium Cantharidate and Sodium Cantharidateon Hep-2 cells.Results:Compared with Sodium Cantharidate,
Magnesium Cantharidate has higher inhibitory effects on the proliferation of Hep-2 cells. The IC50 of Magnesium Cantharidate and the
Sodium Cantharidate was 2.19, 15.75 umol·L-1, respectively. Cell clone forming experiments suggested that the cell colony was difficult
to form when the concentration of Magnesium Cantharidate and the Sodium Cantharidate were 1.75 and 3.50 umol·L-1.Conclusion:Magnesium Cantharidate may have higher inhibitory effects on proliferation of Hep-2 cell than Sodium Cantharidate and has the
development potential in suppressing cancer. |
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