| 王志刚 吕小林 戴革 袁利亚
赵林 胡银英 黄艳琴 袁铿.错配修复基因hMSH2 与慢性粒细胞白血病相关性研究[J].现代生物医学进展英文版,2015,15(12):2225-2229. |
| 错配修复基因hMSH2 与慢性粒细胞白血病相关性研究 |
| Study on the Relationship of Mismatch Repair Gene hMSH2 and ChronicMyeloid Leukemia |
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| DOI： |
| 中文关键词: 慢性粒细胞白血病 错配修复基因 hMSH2 |
| 英文关键词: Chronic myeloid leukemia Mismatch repair gene hMSH2 |
| 基金项目:江西省自然基金项目（20114BAB205021） |
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| 中文摘要: |
| 目的：通过对慢性粒细胞白血病（chronic myeloid leukemia，CML）患者骨髓细胞中错配修复基因（mismatch repair，MMR）
hMSH2的表达水平及其调控机制的分析，探讨hMSH2 与慢性粒细胞白血病疾病进展的联系。方法：用实时定量PCR 方法检测
10 例对照，27 例CML患者（包括慢性期9 例，进展期8 例，急变期10 例）骨髓中4 个MMR 基因(hMSH2、hMSH6、hMLHl、
hPMS2)mRNA的表达；用MSP 方法检测MMR 基因启动子区甲基化水平；用Western blot 方法观察MMR 蛋白水平在各组之间
的差异。结果：与正常对照比较，CML患者的hMSH2 的表达明显降低(P <0.05)，其表达随疾病恶化而下降，依次为急变期＜加速
期＜慢性期，而hMLHl、hPMS2、hMSH6 的表达却未见异常；27 例CML 患者中出现3 例hMSH2 启动子区高甲基化。结论：CML
患者的hMSH2表达水平比正常人显著降低，且随着疾病恶化其表达水平逐下降，提示hMSH2 可能与CML疾病进展相关。 |
| 英文摘要: |
| Objective:To investigate the expression and regulation mechanism of mismatch repair (MMR) gene hMSh2 of bone
marrow in chronic myeloid leukemia (CML), and study the relationship of mismatch repair gene hMSH2 and chronic myeloid leukemia
progression.Methods:Expression of MMR genes hMSH2, hMSH6, hMLHl and hPMS2 mRNAs in 27 CML patients (including chronic
phase 9, accelerated phase 8 and blast crisis 10 cases) and the normal control group (10 cases) were detected by quantitative polymerase
chain reaction (Q-PCR); methyl-specific PCR (MSP) was used to analyze methylation status of hMLH1 and hMSH2 promoters in CML;
the expression of hMSH2 protein was detected by Western blot.Results:The expression of hMSH2 was significantly lower in CML than
in normal control (P <0.05), and decreased gradually with the deterioration of disease(blast crisis |
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