| 郑义彩 徐斌 肖国岫 张任秀 宋亮.短期高浓度氟对小鼠磨牙成釉细胞形态及BMP-4 表达的影响[J].现代生物医学进展英文版,2015,15(11):2034-2036. |
| 短期高浓度氟对小鼠磨牙成釉细胞形态及BMP-4 表达的影响 |
| Effect of Short Exposure to High Levels of Fluoride on Cellular Structure andthe Expression of BMP-4 in Ameloblasts of Mouse Molar |
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| DOI： |
| 中文关键词: 氟化物 成釉细胞 骨形成蛋白-4 |
| 英文关键词: Fluoride Ameloblast BMP-4 |
| 基金项目:上海市第五人民医院青年科研基金项目（2011WYQJ03） |
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| 中文摘要: |
| 目的：研究短期高浓度氟对小鼠磨牙成釉细胞形态及骨形成蛋白-4（BMP-4）表达的影响,探讨氟对牙本质发育的相关机制。
方法：选择40 只4 日龄的ICR小鼠为研究对象，随机分2组各20 只，每组对照鼠与实验鼠各半，对实验动物进行单次腹腔注射，
剂量分别为10mg/kg和20mg/kg的NaF，对照组动物则进行单次腹腔注射等剂量的NaCl，24 小时后处死动物。采用免疫组化染
色观察高浓度氟对小鼠磨牙不同分化阶段成釉细胞形态及BMP-4 表达的影响。结果：成釉细胞在分泌过渡期及早、晚期对短期暴
露高浓度F- 敏感，与对照组小鼠相比，实验组小鼠成熟期成釉细胞无明显变化，过渡期与分泌晚期成釉细胞中BMP-4 的表达显
著降低，差异有统计学意义（P<0.05）。结论：短期高浓度氟对分泌过渡期及晚期成釉细胞中BMP-4 的表达有抑制作用。 |
| 英文摘要: |
| Objective:To investigate the effect of short exposure to high levels of fluoride on cellular structure and the expression
of BMP-4 in ameloblasts of mouse molar, to investigate the related mechanismof Fluoride on the development of dentin.Methods:40 4-
dayold ICR mice were randomly divided into two groups with 20 cases in each one. 10 experimental mice in each group were given
single intraperitoneal injection of 20 mg/kg or 10 mg/kg NaF while the control mice received equal doses of NaCl. All mice were
executed after 24 hours, the structural changes and the expression of BMP-4 in ameloblasts of mouse molar at different stages were
observed by immunohistochemical stainings.Results:Ameloblasts were sensitive to short exposure of high dose F-when they were in the
early,late and transitional secretory stages. Compared with the control group mice,there were no significant changes in maturation
ameloblasts,and the expression of BMP-4 decreased significantly in the late and transitional secretory stage (P<0.05).Conclusion:Short
termexposure of high level of fluoride can suppress the expression of BMP-4 in the late secretory and transitional ameloblast. |
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