| 肖利佳 郝建华 李江 李钢 王娟 邹畅.基因沉默孤儿核受体ERR-alpha抑制前列腺癌细胞的体内转移[J].现代生物医学进展英文版,2015,15(10):1855-1857. |
| 基因沉默孤儿核受体ERR-alpha抑制前列腺癌细胞的体内转移 |
| Knockdown ERR-alpha Inhibits in Vivo Metastasis in Prostate Cancer Cells |
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| DOI： |
| 中文关键词: ERR-alpha E-cadherin 前列腺癌 体内转移 |
| 英文关键词: ERR-alpha E-cadherin Prostate cancer In vivo metastasis |
| 基金项目:广东省深圳市南山科技局项目（2012005） |
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| 中文摘要: |
| 目的：研究孤儿核受体ERR-alpha对前列腺癌细胞E-cadherin(上皮细胞钙粘蛋白)的表达水平和体内转移能力的影响。方法：利
用慢病毒介导的shRNA 构建稳定下调ERR琢表达的DU145-shERR-alpha和PC-3M-shERR-alpha前列腺癌细胞模型，同时用ERR-alpha特异
性抑制剂XCT790 抑制其活性，并利用Western Blotting (免疫印迹) 检测上皮细胞标志物E-cadherin 的表达水平。将
PC-3M-shERR-alpha细胞和PC-3M-scramble 对照细胞用荧光素酶标记后原位注射小鼠前列腺，8 周以后通过体内成像系统检测原位
瘤的形成及其体内转移情况。结果：基因沉默ERR琢表达水平和用其特异性抑制剂XCT790 处理DU145 后，E-cadherin 的表达
水平明显降低。在PC-3M-shERR-alpha细胞中，E-cadherin 的表达水平明显低于对照组，同时由其构建的6 只原位前列腺癌小鼠模型
中没有发生转移，而由对照组细胞构建的7 只原位前列腺癌小鼠模型中有4 只发生了转移。结论：在前列腺癌细胞中下调ERR-alpha
的表达水平抑制其E-cadherin 的表达和体内转移能力。 |
| 英文摘要: |
| Objective:To explore the roles of orphan nuclear receptor ERR-alpha in E-cadherin expression regulation and in vivo
metastasis in prostate cancer cells.Methods:DU145-shERR-alpha and PC-3M-shERR-alpha cell models were established by gene knockdown
mediated by lentivirus. ERR-alpha specific antagnist XCT790 was used to inhibit its activity, the expression level of`E-cadherin was
identified by Western Blotting. PC-3M-shERR-alpha and PC-3M-scramble cells were labeled by luciferase and injected in situ in mice
prostate, metastases were measured through in vivo image system and indicated by the fluorescence intensity 8 weeks after injection.Results:The expression level of E-cadherin was significantly decreased in DU145 after knockdown ERR-alpha or treatment with XCT790.
The expression level of E-cadherin in PC-3M-shERR-alpha was significantly suppressed as compared to that in control cells. Moreover, 4 of
7 in situ prostate cancer mice models dreived from PC-3M-scramble cells, while 0 of 6 from PC-3M-shERR-alpha cells, develop metastases.Conclusion:Knockdown ERR-alpha in prostate cancer cells attenuate E-cadherin expression and their in vivo metastasis. |
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