| 吕晓伟 吴高义 何玉宏 王培欢 朱国雄.慢性睡眠剥夺对颞下颌关节微结构的影响[J].现代生物医学进展英文版,2015,15(10):1840-1843. |
| 慢性睡眠剥夺对颞下颌关节微结构的影响 |
| Effects of Chronic Sleep Deprivation on the Ultrastructure ofTemporomandibular Joint |
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| DOI： |
| 中文关键词: 睡眠障碍 颞下颌关节 髁突软骨 关节盘 微结构 |
| 英文关键词: Sleep disturbance Temporomandibular joint Condylar cartilage Articular disc Ultrastructure. |
| 基金项目:第48 批中国博士后科学基金项目（2010048152）；第4批中国博士后特别资助基金（201104771）；
山东省中青年科学家奖励基金（2011BSBO2001）；济南军区总医院院长基金项目（2010M08） |
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| 中文摘要: |
| 目的：研究慢性睡眠障碍对大鼠颞下颌关节微结构的影响。方法：采用改良多平台法（MMPM）建立睡眠剥夺模型，将90 只
Wistar大鼠随机分为3 组（n=30），分别为小平台组、网格组和对照组。小平台组和网格组大鼠接受每天18 h的睡眠剥夺和6 h间
歇期（10:00—16:00），间歇期大鼠正常笼养。实验第7、14 和21 天时分别行动物行为学观察、旷场试验和动物血浆检测，并通过
HE 染色和扫描电镜观察颞下颌关节微结构的变化。结果：与对照组和网格组相比，小平台组大鼠血清促肾上腺激素（ACTH）和皮
质醇(CORT)水平均增高（P<0.05），髁突软骨HE染色显示软骨细胞层次及厚度改变；扫描电镜结果显示关节盘表面纤维排列松
散。结论：慢性睡眠障碍可能导致颞下颌关节微结构发生病理性改变。 |
| 英文摘要: |
| Objective:To investigate the effects of chronic sleep deprivation (Wistar) on the ultrastructure of temporomandibular
joint (TMJ).Methods:The Sleep deprivation (SD) was conducted according to modified multiple platform method (MMPM). The
platforms were the support points for the animals when the tank filled with water, and rats which were placed on narrow platforms would
fall into the water and wake up when their muscle relaxed (fall into sleep) during sleep deprived period; while rats that stayed on wide
platforms were allowed to lie down without falling into water and could sleep anytime. Food and water were available to the animals at
all times during the experiment. 90 Wistar rats were divided into three groups (N=30), narrow platform group, grid group and control
group. The sleep deprivation protocol was accomplished by sleep depriving with rats for 18 h every day for 3 weeks, and the rats were
allowed to sleep for 6h (from 10:00 to 16:00) in cages. The stress level of animals was estimated by the change of behavior, elevated
plus-maze (EPM) and hormal assays, while the histomorphology and microstructure of rat condyles were observed by hematoxylineosin
(HE) staining and scanning electron microscopy (SEM) at the 1, 2, and 3 week during the experiment, respectively.Results:Both plasma
adrenocorticotropic hormone (ACTH), corticosterone (CORT) were higher in the narrow platform group than that of grid group and
control group (P<0.05). HE staining and histomorphometric data analysis showed decreased thicknesses of the central and posterior
condylar cartilages in stressed rats at the 3 weeks in the narrow platform group. However, the collagen fibers fissures could be observed
on the disc of TMJ under the scanning electron microscope.Conclusion:Sleep deprivation could lead to pathologic changes of the
condyles of stressed rats, suggesting a potential link between sleep disturbance and the pathogenesis or progression of TMD. |
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