| 王鹏 黄晨 麦海星 吴世奎 陈立军.紧密连接蛋白claudin-4 在肾透明细胞癌中的表达及预后意义[J].现代生物医学进展英文版,2015,15(10):1825-1829. |
| 紧密连接蛋白claudin-4 在肾透明细胞癌中的表达及预后意义 |
| Expression of Claudin-4 in Human Clear-cell Renal Cell Carcinoma and itsRelationship with Clinical Pathology and Prognosis |
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| DOI： |
| 中文关键词: 肾透明细胞癌 claudin-4 免疫组织化学 蛋白印记法 无疾病生存期 |
| 英文关键词: Clear-cell renal cell carcinoma Claudin-4 Immunohistochemistry Western blot DFS |
| 基金项目:吴阶平医学基金（32067521203） |
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| 中文摘要: |
| 目的：探讨claudin-4 在肾透明细胞癌中的表达及与临床病理特征及预后的相关性。方法：应用免疫组织化学技术检测50 例
肾透明细胞癌组织及10 例正常肾组织标本中claudin-4 的表达情况，并分析与临床病理参数的相关性；随机选取其中4 例肾透明
细胞癌患者癌组织及4 例正常肾新鲜组织，Western blot 检测claudin-4 的表达情况，并与免疫组化检测结果进行相关性分析；结
合随访结果分析claudin-4 的表达水平与患者无疾病生存期的关系。结果：Claudin-4在肾透明细胞癌组织中的表达显著高于肾正
常组织(P<0.05) ，表达水平与肿瘤的直径、TNM分期、临床分期及淋巴结转移相关；Western blot 结果也表明claudin-4 在肾透明细
胞癌组织中的表达显著高于肾正常组织(P<0.01)，与免疫组化结果呈显著正相关(r2=0.748，P<0.01)；Kaplan-Meier 分析显示
claudin-4 高表达组患者的患者无疾病生存期为20.7 个月，显著低于低表达组的30.8 个月。COX多因素比例风险模型分析显示，
claudin-4 的表达不是肾透明细胞癌的独立预后因素（RR值=1.686，95%CI 0.174~16.311，P=0.652)。结论：claudin-4 蛋白表达上
调可能促进肾透明细胞癌的发生和发展，有可能作为肾透明细胞癌潜在的治疗靶点及影响不良预后的指标。 |
| 英文摘要: |
| Objective:To investigate the expressions of claudin-4 in human clear-cell renal cell carcinoma (ccRCC), and its
relationship with clinical pathology and prognosis.Methods:The expression of claudin-4 in 50 cases of ccRCC and 10 normal kidney
tissues were detected by immunohistochemical staining (IHC), analyzing the relationship between the expression level of claudin-4 and
clinicopathological variables. Western blot was employed to confirm the expression of claudin-4 in 4 cases of ccRCC and 4 normal
kidney tissues. The relationship of claudin-4 expression level and disease-free survival (DFS) was analyzed to predict the prognosis of
ccRCC.Results:The expression level of claudin-4 in ccRCC was significantly higher than that in normal kidney tissues (P<0.05) and was
markedly associated with tumor diameters, T stages, clinical stages and lymph node metastasis. The result of Western blot also confirmed
that the expression of claudin-4 was much higher in ccRCC than normal kidney tissues (P<0.01), which were positively correlated with
the results of immunohistochemical (IHC) staining (r2=0.748, P<0.01). Furthermore, Kaplan-Meier analysis showed that elevated
expression of claudin-4 was associated with worse prognosis in ccRCC. Cox proportional-hazards regression model analysis indicated
that claudin-4 was not an independent prognostic factor for ccRCC patients after surgery(relative risk=1.686, 95%CI:0.174-16.311, P=0.
652).Conclusion:Claudin-4 might play a positive role in carcinogenesis and development of ccRCC, which might become a potential
therapeutic target and adverse prognostic factor. |
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