| 郭争争 程瑞婧 杨丽 王如锋 胡会娟 吴佩根 刘容秀 魏胜利△.不同繁殖模式下甘草药用成分含量积累速度差异研究[J].现代生物医学进展英文版,2015,15(10):1816-1819. |
| 不同繁殖模式下甘草药用成分含量积累速度差异研究 |
| Differences between Different Propagation Modes in the Accumulation Rateof the Content of Medicinal Components in Glycyrrhiza Uralensis Fisch |
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| DOI： |
| 中文关键词: 甘草 繁殖模式 药用成分 HPLC 积累速度 |
| 英文关键词: Licorice Propagation modes Medicinal components HPLC Accumulation rate |
| 基金项目:北京市自然科学基金项目（7122094） |
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| 中文摘要: |
| 目的：系统比较野生甘草无性繁殖的甘草根和人工甘草种子有性繁殖的甘草根中药用成分的积累速度差异情况，为进一步
研究影响甘草药用成分积累的基因机制奠定理论基础。方法：本研究基于HPLC 和UV等分析方法，比较野生甘草和人工甘草在
甘草酸、甘草苷和甘草总黄酮以及其他类成分的含量差异。基于HPLC 指纹图谱技术，全面分析不同繁殖模式下甘草根的化学信
息和内在质量的差异情况。结果：除甘草素和异甘草素外，野生甘草不定根中其他三种药用成分和总黄酮的含量均高于人工甘草
实生根，经SPSS 分析，除异甘草素外，其他成分在两组之间均存在显著差异；根据HPLC 指纹图谱，两种甘草药用成分之间差异
显著，相似度为0.647。结论：野生无性繁殖的甘草根和人工有性繁殖的甘草根中主要药用成分含量之间存在显著差异，野生甘草
具有快速积累药用成分的功能。本研究结果对于今后人工调控生产优质甘草药材，实现甘草资源的可持续利用具有重要的理论
和实践意义。 |
| 英文摘要: |
| Objective:To systematically compare the difference in the accumulation rate of the content of medicinal components
between licorice roots formed by asexual reproduction of wild licorice and sexual reproduction of cultivated licorice. Accordingly, this
could be the theoretical basis to further study genetic mechanisms influencing the accumulation of medicinal components.Methods:In
this study the contents of Glycyrrhizin, Liquiritin and total flavonoids and other types of components in wild and cultivated roots were
analyzed by HPLC and UV; And based on HPLC fingerprint technology, the differences in the chemical information and the quality of
licorice roots under different propagation modes were comprehensively analyzed.Results:Except for the contents of liquiritigenin and
isoliquiritigenin, the contents of other three medicinal components and total flavonoids in wild root formed by asexual reproduction are
higher than those in cultivated root formed by sexual reproduction. And all the contents of medicinal components but for isoliquiritigenin
were different significantly by SPSS analysis; According to HPLC fingerprint, there were significant differences between wild and
cultivated roots, and there was a low similarity of 0.647.Conclusion:There are significant differences between licorice roots formed by
asexual reproduction of wild licorice and sexual reproduction of cultivated licorice in the accumulation rates of the content of medicinal
components. And wild licorice has the function to accumulate the contents of medicinal components rapidly. The results could be of
important theoretical and practical significance to regulate the production of high quality artificial licorice herbs and realize the
sustainable utilization of licorice resource in the future. |
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